Genotyping of Acanthamoeba in Rural Drinking Water Sources in Kashan and Aran-Bidgol, Iran

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Mostafaei Gholamreza; Sabbaghian Bidgoli Mansooreh; RASTI SIMA; Moosavi Sayyed Gholamabbas

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:28 | Issue:169 Page(s): 130-139

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Information Journal Paper

Title

Genotyping of Acanthamoeba in Rural Drinking Water Sources in Kashan and Aran-Bidgol, Iran

Author(s)

Mostafaei Gholamreza | Sabbaghian Bidgoli Mansooreh | RASTI SIMA | Moosavi Sayyed Gholamabbas | Issue Writer Certificate

Keywords

free-living amoebaQ1

AcanthamoebaQ2

drinking water sourcesQ2

Abstract

Background and purpose Acanthamoeba spp. are an opportunistic protozoan in environmental sources that can cause respiratory infection and keratitis. The present study was conducted to determine the prevalence and genotypes of Acanthamoeba in rural drinking water sources in Kashan and Aran-Bidgol, Iran. Materials and methods In this study, 162 samples from 54 drinking water sources were collected in 2017. After samples filtration, they were cultured on non-nutrient agar to isolate free-living amoeba. After DNA extraction, the Polymerase Chain Reaction using JDP1, JDP2 primers was performed to detect Acanthamoeba spp. and finally the genotype of eight isolates was determined. Data analysis was done in SPSS applying Chi-square and Fisher’ s exact test. Results Among the samples 35. 2% were found to be contaminated with free-living amoeba, among which 19 isolates (11. 7%) were confirmed as Acanthamoeba. The rates of free-living amoeba and Acanthamoba were 35. 4% and 11. 8%, respectively, in Kashan, which were higher than those in Aran-Bidgol. Frequency of free-living amoeba in Qanat, well, and spring was 55. 6%, 30. 2%, and 26. 7%, respectively. Acanthamoeba contamination rates were 13. 9%, 11. 5% and 10%, respectively. Acanthamoeba contamination rate was higher in surface wells than that in deep wells. The genotype of all Acanthamoeba isolates belonged to T4. Conclusion This study revealed that drinking water sources were contaminated with free-living amoeba and Acanthamoeba. All isolates were T4 genotype, therefore, these sources could be considered as a risk factor for public health.

Cites

Survey on the Contamination Rate of Stagnant Water and Soil to Vermamoeba vermiformis in Arak Parks, Iran, 2017-2018

References

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APA: Copy

Mostafaei, Gholamreza, Sabbaghian Bidgoli, Mansooreh, RASTI, SIMA, & Moosavi, Sayyed Gholamabbas. (2019). Genotyping of Acanthamoeba in Rural Drinking Water Sources in Kashan and Aran-Bidgol, Iran. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(169 ), 130-139. SID. https://sid.ir/paper/46121/en

Vancouver: Copy

Mostafaei Gholamreza, Sabbaghian Bidgoli Mansooreh, RASTI SIMA, Moosavi Sayyed Gholamabbas. Genotyping of Acanthamoeba in Rural Drinking Water Sources in Kashan and Aran-Bidgol, Iran. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;28(169 ):130-139. Available from: https://sid.ir/paper/46121/en

IEEE: Copy

Gholamreza Mostafaei, Mansooreh Sabbaghian Bidgoli, SIMA RASTI, and Sayyed Gholamabbas Moosavi, “Genotyping of Acanthamoeba in Rural Drinking Water Sources in Kashan and Aran-Bidgol, Iran,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 169 , pp. 130–139, 2019, [Online]. Available: https://sid.ir/paper/46121/en

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