MORPHOMETRICAL STUDY OF MOUSE PRE-IMPLANTATION EMBRYOS: COMPARISON OF IN VITRO- AND IN VIVO-PRODUCED EMBRYOS

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KARIMPOUR MALEKSHAH A.A.; BAHR ALOLOUMI S.; KALTEH A.; ESMAEILNEZHAD MOGHADAM A.; TALEBPOUR FERESHTEH

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Journal: Journal of Mazandaran University of Medical Sciences Year:2009 | Volume:18 | Issue:67 Page(s): 43-49

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Title

MORPHOMETRICAL STUDY OF MOUSE PRE-IMPLANTATION EMBRYOS: COMPARISON OF IN VITRO- AND IN VIVO-PRODUCED EMBRYOS

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Keywords

MORPHOMETRYQ3

MOUSE EMBRYOQ3

BLASTOCYSTQ3

ZONA PELLUCIDAQ3

Abstract

Background and purpose: The development of pre-implantation mammalian embryos in vitro is compromised, compared with those grown in vivo. Selecting embryos with a high implantation potential is one of the most important challenges in the field of assisted reproductive technology. The aim of this study was to postulate morphometrical characteristics of good quality embryos, with comparisons between in vivo and in vitro produced MOUSE EMBRYOs.Materials and methods: Embryos was obtained from NMRI female mice after super ovulation. In vivo developed 2-, 4- and 8-cell embryos; morulla and full BLASTOCYST were isolated from mice on 18, 36, 52, 60, 72 and 96 hours after hCG administration respectively. Ham, s F10 medium was used for in vitro culture of embryos. External and internal diameter of embryos, zona thickness and number of cells in full BLASTOCYSTs were evaluated and compared between in vivo and in vitro groups.Results: External and internal diameter and zone thickness in oocyte and zygotes were 99.9 mm, 75.4 mm and 4.9 mm respectively. These values did not change prior to the BLASTOCYST stage in both in vivo and in vitro groups; but in full BLASTOCYST stage, the diameter of embryos significantly increased and zone thickness decreased compared to prior stages in both groups (P<0.01). The diameter of full BLASTOCYSTs of in vivo group (116.5 mm) were significantly larger than those of in vitro group (104.3 mm, P<0.05). Moreover, the full BLASTOCYSTs of in vivo group had significantly more blastomeres (49), compared to in vitro group (43, P<0.05). Additionally, cultured embryos reached full BLASTOCYST at 110 hours after hCG administration, while in vivo condition the time frame was 96 hours.Conclusion: Based on the above results, embryo size and zona thickness cannot predict embryo quality prior to BLASTOCYST stage, however, in this stage; larger embryos and those that have more blastomere may show greater viability.

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APA: Copy

KARIMPOUR MALEKSHAH, A.A., BAHR ALOLOUMI, S., KALTEH, A., ESMAEILNEZHAD MOGHADAM, A., & TALEBPOUR, FERESHTEH. (2009). MORPHOMETRICAL STUDY OF MOUSE PRE-IMPLANTATION EMBRYOS: COMPARISON OF IN VITRO- AND IN VIVO-PRODUCED EMBRYOS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 18(67), 43-49. SID. https://sid.ir/paper/46212/en

Vancouver: Copy

KARIMPOUR MALEKSHAH A.A., BAHR ALOLOUMI S., KALTEH A., ESMAEILNEZHAD MOGHADAM A., TALEBPOUR FERESHTEH. MORPHOMETRICAL STUDY OF MOUSE PRE-IMPLANTATION EMBRYOS: COMPARISON OF IN VITRO- AND IN VIVO-PRODUCED EMBRYOS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2009;18(67):43-49. Available from: https://sid.ir/paper/46212/en

IEEE: Copy

A.A. KARIMPOUR MALEKSHAH, S. BAHR ALOLOUMI, A. KALTEH, A. ESMAEILNEZHAD MOGHADAM, and FERESHTEH TALEBPOUR, “MORPHOMETRICAL STUDY OF MOUSE PRE-IMPLANTATION EMBRYOS: COMPARISON OF IN VITRO- AND IN VIVO-PRODUCED EMBRYOS,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 18, no. 67, pp. 43–49, 2009, [Online]. Available: https://sid.ir/paper/46212/en

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