MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD

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REZVANI GILKOLAEI S.; BABAEI S.A.; POURKAZEMI MOHAMMAD

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Journal: Iranian Scientific Fisheries Journal Year:2001 | Volume:10 | Issue:2 Page(s): 15-30

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Title

MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD

Author(s)

REZVANI GILKOLAEI S. | BABAEI S.A. | POURKAZEMI MOHAMMAD | Issue Writer Certificate

Keywords

PENAEUS SEMISULCATUSQ4

CYTECHROMQ4

COLQ4

RFLPQ4

PERSIAN GULFQ4

IRANQ4

Abstract

The 0bjective of this investigation was molecular population study on PENAEUS SEMISULCATUS stocks from the PERSIAN GULF and Oman Sea. Samples were COLlected using trawling method from Hormouz (40 individuals) and Bushehr (35 individuals) regions.The DNA of samples were extracted using Phenol and chloroform method and then were amplified using a pair primer of Cytochrom Oxidase subunit I (COI) gene sequences by a thermal cycler. Nine restriction enzyme were used to digest the target gene region that five of them (AluI, HinfI, HincII, HpaII and RsaI) appeared Polymorphic patterns. Reap software and X2 test were used to analyse the RFLP data.The average of nucleotide diversity and haplotype diversity among the population were 0.0345720±0.0011952 and 0.28590±0.08174 and nucleotide divergence among population, being studied, is supposed to be 8.5%. Considering the results, dispersion of haplotypes in two regions showed a significant difference and this is an evidence for proving the variety of the stocks.The minimum and maximum biomass of zooplanktons and benthic organisms in the ponds treated with fertilizer and live-food were estimated 0.018 and 38.63 g/m3; 0.0 to 2.1 g/m2, respectively. No significant difference was observed in the productivity of the ponds. On the basis of the abundance of prey in the studied ponds, D. magna and D. pulex were the primary prey (Fp>50), cyclops and tlauplii of cyclops were secondary prey (10< Fp<50) and Tubificidae and Chironomidae were the occasional preys (Fp=O). The results of GSI observations indicated a decrease in daily growth rate and condition factor with an increase in weight and length of the fingerlings, but this difference was not significam. The analysis of gut contents of the 120 fingerlings in the ponds treated with animal fertilizer and live-food indicated that 30 of the fingerlings were reared under good rearing conditions

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APA: Copy

REZVANI GILKOLAEI, S., BABAEI, S.A., & POURKAZEMI, MOHAMMAD. (2001). MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL, 10(2), 15-30. SID. https://sid.ir/paper/4743/en

Vancouver: Copy

REZVANI GILKOLAEI S., BABAEI S.A., POURKAZEMI MOHAMMAD. MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD. IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2001;10(2):15-30. Available from: https://sid.ir/paper/4743/en

IEEE: Copy

S. REZVANI GILKOLAEI, S.A. BABAEI, and MOHAMMAD POURKAZEMI, “MOLECULAR POPULATION STUDY ON PENAEUS SEMISULCATUS FROM THE PERSIAN GULF AND OMAN SEA USING CYTOCHROM OXIDASE SUBUNIT I (COL) GENE BY RFLP METHOD,” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 10, no. 2, pp. 15–30, 2001, [Online]. Available: https://sid.ir/paper/4743/en

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