مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

1,254
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

IDENTIFICATION OF BRENNERIA NIGRIFLUENS ISOLATES

Pages

  263-277

Abstract

 The shallow bark canker of Persian walnut (Juglans regia L.) trees incited by the bacterium Brenneria nigrifluens affects the bark of trunk and scaffold branches. Fifty-nine isolates of bacterium were isolated on the basis of disease symptoms from 114 plant samples, collected from Mazandaran, Golestan, Kerman and Kermanshah provinces. Furthermore, 10 isolates from Fars province, one isolate from Kohgiluyeh-va-Boyerahmad province and one isolate from Kurdistan province were received. Based on physiological and biochemical characters, bacterial isolates were divided into two groups and identified as Brenneria nigrifluens or strains close to B. nigrifluens. The electrophoretic profile of the strains cell proteins also confirmed this grouping. Isolates were assessed by polymerase chain reaction (PCR) using a specific primer pair for B. nigrifluens. Twenty four isolates produced the expected band of approximately 255 bp and were named PCR+ strains. Pathogenicity tests performed with one PCR+ isolate and three PCR- isolates showed that only PCR+ isolate was able to cause cankers in walnut seedlings. Eight weeks after inoculation of PCR+ isolates on Persian walnut seedlings, water-soaked cankers appeared around the inoculation sites. The bacterium was reisolated from the bark lesions of the inoculated seedling on EMB-agar medium. To investigate the certitude of the primer pair, one PCR+ isolate and three PCR- isolates from different geographic locations were analyzed by sequencing 16S RDNA. The results revealed that PCR+ isolates were Brenneria nigrifluens. On the basis of this study, it can be concluded that general phenotypic tests are not reliable enough to identify B. nigrifluens isolates and that specific detection of the pathogen with pathogenicity test or PCR is necessary.

Cites

  • No record.
  • References

  • No record.
  • Cite

    APA: Copy

    FALAHI CHARKHABI, N., SHAMS BAKHSH, M., RAHIMIAN, H., & KHODAYEGAN, P.. (2011). IDENTIFICATION OF BRENNERIA NIGRIFLUENS ISOLATES. IRANIAN JOURNAL OF PLANT PATHOLOGY, 47(3 (187)), 263-277. SID. https://sid.ir/paper/48165/en

    Vancouver: Copy

    FALAHI CHARKHABI N., SHAMS BAKHSH M., RAHIMIAN H., KHODAYEGAN P.. IDENTIFICATION OF BRENNERIA NIGRIFLUENS ISOLATES. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2011;47(3 (187)):263-277. Available from: https://sid.ir/paper/48165/en

    IEEE: Copy

    N. FALAHI CHARKHABI, M. SHAMS BAKHSH, H. RAHIMIAN, and P. KHODAYEGAN, “IDENTIFICATION OF BRENNERIA NIGRIFLUENS ISOLATES,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 47, no. 3 (187), pp. 263–277, 2011, [Online]. Available: https://sid.ir/paper/48165/en

    Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    Move to top