STUDY OF GENETIC DIVERSITY OF IRANIAN ISOLATES OF RHIZOCTONIA SOLANI AG1-IA USING RAPD MARKERS

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KHODAYARI M.; SAFAIE N.; SHAMS BAKHSH M.

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Paper Information

Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2008 | Volume:44 | Issue:3 (175) Page(s): 215-231

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Title

STUDY OF GENETIC DIVERSITY OF IRANIAN ISOLATES OF RHIZOCTONIA SOLANI AG1-IA USING RAPD MARKERS

Author(s)

KHODAYARI M. | SAFAIE N. | SHAMS BAKHSH M. | Issue Writer Certificate

Keywords

SHEATH BLIGHTQ3

RHIZOCTONIA SOLANI AG1-IAQ3

RAPDQ2

28S RDNAQ3

IRANQ2

Abstract

Rhizoctonia solani AG1-IA (teleomorph: Thanatephorus cucumeris), is the causal agent of SHEATH BLIGHT disease of rice in northern paddies of IRAN. Due to the importance of this disease and the lack of knowledge on genetic diversity of its causative agent in IRAN, 25 isolates were collected from Guilan (23 isolates) and Mazandaran (two isolates) provinces and examined for their morphological characteristics and genetic diversity. This study showed that all isolates were AG1-IA and further confirmed using AG1-IA specific primers for 10 selected isolates which amplified a single band of 265bp. No band was amplified in AG4 as negative control. The results of colony growth rate divided the isolates into high (68% of isolates) and low (32% of isolates) growth rate categories. Genetic diversity was estimated among all isolates, using RAPD analysis. Seven out of 20 random primers showed reproducible polymorphic banding pattern. The size of amplified DNA bands ranged from 0.25 to 5Kb. Cluster analysis of the resulting data was performed using UPGMA method, Jaccard’s coefficient in NTSYS-pc software. The resulting dendrogram categorized the isolates into two groups of high and low growth rate at 34% similarity level. At 80% similarity level, the isolates were categorized into 23 groups which indicate a high genetic diversity of rice SHEATH BLIGHT fungus revealed by RAPD primers. The result of molecular analysis showed that there was no correlation between geographical origins of isolates and the resulting genetic diversity patterns. This is the first study on direct identification of R. solani AG1-IA by specific primers and evolution of genetic diversity of rice SHEATH BLIGHT fungus using RAPD marker in IRAN.

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APA: Copy

KHODAYARI, M., SAFAIE, N., & SHAMS BAKHSH, M.. (2008). STUDY OF GENETIC DIVERSITY OF IRANIAN ISOLATES OF RHIZOCTONIA SOLANI AG1-IA USING RAPD MARKERS. IRANIAN JOURNAL OF PLANT PATHOLOGY, 44(3 (175)), 215-231. SID. https://sid.ir/paper/48278/en

Vancouver: Copy

KHODAYARI M., SAFAIE N., SHAMS BAKHSH M.. STUDY OF GENETIC DIVERSITY OF IRANIAN ISOLATES OF RHIZOCTONIA SOLANI AG1-IA USING RAPD MARKERS. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2008;44(3 (175)):215-231. Available from: https://sid.ir/paper/48278/en

IEEE: Copy

M. KHODAYARI, N. SAFAIE, and M. SHAMS BAKHSH, “STUDY OF GENETIC DIVERSITY OF IRANIAN ISOLATES OF RHIZOCTONIA SOLANI AG1-IA USING RAPD MARKERS,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 44, no. 3 (175), pp. 215–231, 2008, [Online]. Available: https://sid.ir/paper/48278/en

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