CONFERRING RESISTANCE TO PLRV IN TRANSGENIC TOBACCO PLANTS THROUGH THE INDUCTION OF POST TRANSCRIPTIONAL GENE SILENCING

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NIKAN J.; BARKER H.

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Journal: IRANIAN JOURNAL OF PLANT PATHOLOGY Year:2007 | Volume:43 | Issue:3 (171) Page(s): 325-326

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Title

CONFERRING RESISTANCE TO PLRV IN TRANSGENIC TOBACCO PLANTS THROUGH THE INDUCTION OF POST TRANSCRIPTIONAL GENE SILENCING

Author(s)

NIKAN J. | BARKER H. | Issue Writer Certificate

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Abstract

Two DNA fragments of a Potato leafroll virus full-length cDNA which comprised parts of the PLRV ORF2 sequence were amplified by PCR. Both fragments were exactly the same except that they had the restriction sites for the enzymes XbaI and KpnI at the opposite ends.Therefore insertion of the two fragments into the same binary plasmid transformation vector would take place in the opposite orientations (sense or antisense). Using an Agrobacterium tumefaciens-mediated plant transformation procedure, leaf discs of Nicotiana tabacum were transformed with either of the fragments and regenerated into whole plants. Using PCR and RT-PCR, the transgenic plants that expressed mRNA transcripts of the transgenes were identified. Six lines of the plants transformed with sense and six lines of those transformed with antisense were crossed and the remaining was allowed to self-fertilise. The seeds obtained from these were grown to produce seedlings used in resistance tests. The resistance tests were done by aphid inoculation of seedlings with PLRV. Two weeks later the inoculated seedlings were tested for infection with PLRV by ELISA. Of the lines transgenic for sense and antisense only 28.5% and 23% were resistant to PLRV accumulation, respectively, whereas 50% of the lines obtained from crosses between sense and antisense plants were resistant. It seems that the simultaneous expression of sense plus antisense mRNA could have triggered post transcriptional gene silencing more effectively than expression of either sense or antisense mRNA alone.

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APA: Copy

NIKAN, J., & BARKER, H.. (2007). CONFERRING RESISTANCE TO PLRV IN TRANSGENIC TOBACCO PLANTS THROUGH THE INDUCTION OF POST TRANSCRIPTIONAL GENE SILENCING. IRANIAN JOURNAL OF PLANT PATHOLOGY, 43(3 (171)), 325-326. SID. https://sid.ir/paper/48347/en

Vancouver: Copy

NIKAN J., BARKER H.. CONFERRING RESISTANCE TO PLRV IN TRANSGENIC TOBACCO PLANTS THROUGH THE INDUCTION OF POST TRANSCRIPTIONAL GENE SILENCING. IRANIAN JOURNAL OF PLANT PATHOLOGY[Internet]. 2007;43(3 (171)):325-326. Available from: https://sid.ir/paper/48347/en

IEEE: Copy

J. NIKAN, and H. BARKER, “CONFERRING RESISTANCE TO PLRV IN TRANSGENIC TOBACCO PLANTS THROUGH THE INDUCTION OF POST TRANSCRIPTIONAL GENE SILENCING,” IRANIAN JOURNAL OF PLANT PATHOLOGY, vol. 43, no. 3 (171), pp. 325–326, 2007, [Online]. Available: https://sid.ir/paper/48347/en

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