PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST SHEEP RED BLOOD CELLS (SRBC) IN RABBIT (HEMOLYSIN)

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MAJIDI J.; BANAZADEH AMIRKHIZ M.

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Journal: PHARMACEUTICAL SCIENCES Year:2004 | Volume:- | Issue:2 Page(s): 69-74

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Title

PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST SHEEP RED BLOOD CELLS (SRBC) IN RABBIT (HEMOLYSIN)

Author(s)

MAJIDI J. | BANAZADEH AMIRKHIZ M. | Issue Writer Certificate

Keywords

POLYCLONAL ANTIBODIESQ3

SHEEP RED BLOOD CELLS (SRBC)Q3

Abstract

Hemolysinhas various diagnostic as well as academic applications. Apart from the regular teaching of this test to postgraduate students of different fields like medicine, pharmacy, immunology and biochemistry, the assessment of complement components' function by performing CH50 test is one of its applicability. The goal of this project was to take a step towards self-efficiency with a minimum expense. Hemolysin was produced in a rabbit by using sheep red blood cells (SRBC) as immunogenic. Heparinized blood from several sheeps were mixed together (pooled) and then washed with serum physiology. The number of red blood cells was counted with a cell counter, and 1-5x109 numbers of red cells were injected intravenously into the rabbit. The production of POLYCLONAL ANTIBODIES of IgM class reached its maximumlevel after seven to ten days of injection. The blood was withdrawn from the rabbit and its serum, rich in hemolysin, was separated. Hemolysin was titrated for assessment of the hemolysin function and its standardization. To do this and determining in which titer of hemolysin red blood cells only become sensitive and not agglutinated, different dilutions of hemolysin were made, and added to certain volumes of SRBC. The results showed that 2560 dilution of hemolysin was still capable of sensitizing SRBC for surveying function of total complement components. To be sure of hemolysin function at a standard level, CH50 test was performed using test and standard hemolysin at the same time. The results showed that 2560 dilution of test hemolysin, the same as standard hemolysin was capable of making sheep red blood cells sensitive. High titer of test hemolysin in comparison with titer of standard hemolysinclearly indicates that hemolysin produced in our laboratory is equal in potential to the standard. Thus it is a beneficial production and has importance for diagnostic purposes.

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APA: Copy

MAJIDI, J., & BANAZADEH AMIRKHIZ, M.. (2004). PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST SHEEP RED BLOOD CELLS (SRBC) IN RABBIT (HEMOLYSIN). PHARMACEUTICAL SCIENCES, -(2), 69-74. SID. https://sid.ir/paper/48481/en

Vancouver: Copy

MAJIDI J., BANAZADEH AMIRKHIZ M.. PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST SHEEP RED BLOOD CELLS (SRBC) IN RABBIT (HEMOLYSIN). PHARMACEUTICAL SCIENCES[Internet]. 2004;-(2):69-74. Available from: https://sid.ir/paper/48481/en

IEEE: Copy

J. MAJIDI, and M. BANAZADEH AMIRKHIZ, “PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST SHEEP RED BLOOD CELLS (SRBC) IN RABBIT (HEMOLYSIN),” PHARMACEUTICAL SCIENCES, vol. -, no. 2, pp. 69–74, 2004, [Online]. Available: https://sid.ir/paper/48481/en

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