SIMULTANEOUS EXPRESSION OFLNTERLEUKIN-2 AND B7.1 USING A RECOMBINANT ADENOVIRUS VECTOR WITH A SINGLE EXPRESSION CASSETTE CONTAINING IRES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HEJAZI M.S.; FARZANEH F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: PHARMACEUTICAL SCIENCES Year:2003 | Volume:- | Issue:1 Page(s): 83-97

Download Full-Text

Persian Verion

View:

1,628

Download:

Cites:

Information Journal Paper

Title

SIMULTANEOUS EXPRESSION OFLNTERLEUKIN-2 AND B7.1 USING A RECOMBINANT ADENOVIRUS VECTOR WITH A SINGLE EXPRESSION CASSETTE CONTAINING IRES

Author(s)

HEJAZI M.S. | FARZANEH F. | Issue Writer Certificate

Keywords

RECOMBINANT ADENOVIRUSQ3

VECTORQ3

EXPRESSIONQ3

INTERLEUKIN-2Q3

B7.1Q3

INTERNAL REBOSOME ENTRY SITE (IRES)Q3

Abstract

Immunogene therapy is one of the recently developed approaches for the potential treatment of leukaemia based on the leukaemic cells engineered to express immune stimulates and returning them to the patient after irradiation. In this case returned cells are expected to act as a vaccine and stimulate the immune system, specially T Iymphocytes. Experiments have shown that EXPRESSION of either IL-2 or B7.1 molecules have curative effect in murine leukaemia models. Recently, further studies showed that EXPRESSION of IL-2 and B7.1 molecules together is more curative and reject the neoplastic cells resulting in the survival of up to 100% of the mice. This study aimed to evaluate the potency of RECOMBINANT ADENOVIRUS IL-2/IRES-B7.1 in EXPRESSION of two IL-2 and B7.1 molecules simultaneously driven by one promoter and using IRES (internal ribosome entry site) sequence. Adenovirus GFP was used as a marker VECTOR to evaluate the potency of RECOMBINANT ADENOVIRUSes in infection of the cells and induction of the transgene in mouse leukaemic C1498 cells. Adenovirus delta, a similar VECTOR but without the two genes, was used as the control VECTOR. The EXPRESSION of B7.1 molecule on the surface of the cells was determined by FACS analysis and secretion of IL-2 was evaluated by ELISA. The results showed that adenovirus GFP can transducer C1498 and induce EXPRESSION of GFP in these cells. This result indicates that adenovirus VECTORs can transduce C1498 cells. Adenovirus IL-2/IRESIB7.1 VECTOR infection induces the EXPRESSION of B7.1 on the surface of about 38% of the cells and also secretion of IL-2 at about 150 pg/m1/106 cells/24 hours by the transduced cells. As the conclusion it can be said that two IL-2 and B7.1 gene can be expressed using a single EXPRESSION cassette with employing IRES. Additionally, adenovirus VECTORs transduce C1498 cells and adenovirus IL-2/IRES/B7.1 VECTOR is able to induce the EXPRESSION and secretion of B7.1 and IL-2 respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

HEJAZI, M.S., & FARZANEH, F.. (2003). SIMULTANEOUS EXPRESSION OFLNTERLEUKIN-2 AND B7.1 USING A RECOMBINANT ADENOVIRUS VECTOR WITH A SINGLE EXPRESSION CASSETTE CONTAINING IRES. PHARMACEUTICAL SCIENCES, -(1), 83-97. SID. https://sid.ir/paper/48708/en

Vancouver: Copy

HEJAZI M.S., FARZANEH F.. SIMULTANEOUS EXPRESSION OFLNTERLEUKIN-2 AND B7.1 USING A RECOMBINANT ADENOVIRUS VECTOR WITH A SINGLE EXPRESSION CASSETTE CONTAINING IRES. PHARMACEUTICAL SCIENCES[Internet]. 2003;-(1):83-97. Available from: https://sid.ir/paper/48708/en

IEEE: Copy

M.S. HEJAZI, and F. FARZANEH, “SIMULTANEOUS EXPRESSION OFLNTERLEUKIN-2 AND B7.1 USING A RECOMBINANT ADENOVIRUS VECTOR WITH A SINGLE EXPRESSION CASSETTE CONTAINING IRES,” PHARMACEUTICAL SCIENCES, vol. -, no. 1, pp. 83–97, 2003, [Online]. Available: https://sid.ir/paper/48708/en

Related Journal Papers