RAPID DETECTION OF CLARITHROMYCIN-RESISTANT HELICOBACTER PYLORI IN DYSPEPTIC PATIENTS BY FLUORESCENT IN SITU HYBRIDIZATION (FISH) COMPARED WITH E-TEST

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MOUSAVIAN SEYED MOJTABA; TAJBAKHSH S.; SAMARBAFZADEH A.R.

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Paper Information

Journal: Journal of Inflammatory Diseases Year:2006 | Volume:10 | Issue:3 (40) Page(s): 70-75

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Title

RAPID DETECTION OF CLARITHROMYCIN-RESISTANT HELICOBACTER PYLORI IN DYSPEPTIC PATIENTS BY FLUORESCENT IN SITU HYBRIDIZATION (FISH) COMPARED WITH E-TEST

Author(s)

MOUSAVIAN SEYED MOJTABA | TAJBAKHSH S. | SAMARBAFZADEH A.R. | Issue Writer Certificate

Keywords

HELICOBACTER PYLORIQ3

HIBRIDIZATIONQ2

CLARITHROMYCINQ2

PEPTIC ULCERQ3

DIGESTIONQ3

Abstract

Background: Rapid detection of H. pylori is important for determining of resistant are susceptible strains to CLARITHROMYCIN and recovery of patients will be acelerated, if CLARITHROMYCIN is added to therapeutic protocol.Objective: Rapid detection of susceptible or resistant strains of HELICOBACTER PYLORI to CLARITHROMYCIN in patients with dyspeptic ulcers by FISH technique and also comparison of FISH results with E-test technique. Methods: Frozen sections of gastric biopsies from 50 patients with dyspeptic ulcers were hybridized in situ with 5 fluorescent oligonucleotide probes (FISH). Following staining with DAPI, the slides were examined using fluorescent microscopy. Also, susceptibility and resistance of isolated strains of H. pylori to CLARITHROMYCIN was determined by E-test. The results obtained from both E-test and FISH techniques were compared.Findings: Twenty five out of 50 examined gastric biopsy samples were positive for H. pylori by FISH. Out of 25 H. pylori strains, 17 strains (68%) were susceptible, 6 strains (24%) resistant and 2 strains (8%) showed intermediate response to CLARITHROMYCIN. This study showed that there was no significant difference between FISH and E-test results in terms of the number of susceptible or resistance strains.Conclusion: Regarding the results of this study, it sems that the FISH technique to be a suitable method to determine the susceptibility are H. pylori to CLARITHROMYCIN, especially when a quickly decision is necessary for treating of dyspeptic patients.

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APA: Copy

MOUSAVIAN, SEYED MOJTABA, TAJBAKHSH, S., & SAMARBAFZADEH, A.R.. (2006). RAPID DETECTION OF CLARITHROMYCIN-RESISTANT HELICOBACTER PYLORI IN DYSPEPTIC PATIENTS BY FLUORESCENT IN SITU HYBRIDIZATION (FISH) COMPARED WITH E-TEST. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), 10(3 (40)), 70-75. SID. https://sid.ir/paper/49210/en

Vancouver: Copy

MOUSAVIAN SEYED MOJTABA, TAJBAKHSH S., SAMARBAFZADEH A.R.. RAPID DETECTION OF CLARITHROMYCIN-RESISTANT HELICOBACTER PYLORI IN DYSPEPTIC PATIENTS BY FLUORESCENT IN SITU HYBRIDIZATION (FISH) COMPARED WITH E-TEST. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2006;10(3 (40)):70-75. Available from: https://sid.ir/paper/49210/en

IEEE: Copy

SEYED MOJTABA MOUSAVIAN, S. TAJBAKHSH, and A.R. SAMARBAFZADEH, “RAPID DETECTION OF CLARITHROMYCIN-RESISTANT HELICOBACTER PYLORI IN DYSPEPTIC PATIENTS BY FLUORESCENT IN SITU HYBRIDIZATION (FISH) COMPARED WITH E-TEST,” JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), vol. 10, no. 3 (40), pp. 70–75, 2006, [Online]. Available: https://sid.ir/paper/49210/en

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