BIOINFORMATIC DESIGNING FOR PRODUCING VACCINE PEPTIDE OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A), AND EVALUATION OF POLYCLONAL ANTIBODIES IN MICE

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SOLTANPOUR GHARIBDOUSTI FAEZEH; KARDAR GHOLAM ALI; FAZELI DELSHAD BANAFSHEH; FALAK REZA; GANJALIKHANI HAKEMI MAZDAK; ANDALIB ALIREZA

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2016 | Volume:34 | Issue:398 Page(s): 1054-1059

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Title

BIOINFORMATIC DESIGNING FOR PRODUCING VACCINE PEPTIDE OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A), AND EVALUATION OF POLYCLONAL ANTIBODIES IN MICE

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Keywords

BIOINFORMATIC; VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF); PEPTIDE VACCINEQ1

Abstract

Background: It is stated that in the absence of angiogenesis, the tumoral tissue will not grow beyond 2 mm3. Vascular endothelial growth factor (VEGF) plays a pivotal role in angiogenesis and blockade of this process could be applied as a novel strategy for immunotherapy of cancerMethods: Peptide sequences of VEGF-A isoforms were retrieved from protein databases and aligned. Immunodominant epitopes were determined and the selected one was rechecked for dissimilarity with other human proteins. The selected conserved peptide sequence was synthesized and conjugated with Keyhole limpet hemocyanin (KLH). Then, it was applied for immunization of mice. The polyclonal anti-VEGF antibody titer was measured using an indirect peptide-enzyme-linked immunosorbent assay (ELISA) with a Bovine serum albumin (BSA)-conjugated peptide.Findings: According to bioinformatic findings, the selected 41-aminoacid sequence did not show any similarity with other human proteins and revealed enough antigenicity to stimulate anti-tumor specific responses. A substantial increase of specific antibody titer was observed in vaccinated mice. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the BSA-conjugated peptide showed efficient coupling of the molecules. Optimization steps in ELISA procedures revealed that coating of microtiter plates with BSA-conjugated antigen provided more reproducible outcome than unconjugated peptide.Conclusion: Our results reinforce the potential of KLH-conjugated peptides for immunization and production of specific polyclonal antibodies against VEGF-A. Production of high-titer antibodies against this antigen indicates that the designed peptide-vaccine could be used as a potential immunogen for stimulation of humoral immune system in animal model.

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APA: Copy

SOLTANPOUR GHARIBDOUSTI, FAEZEH, KARDAR, GHOLAM ALI, FAZELI DELSHAD, BANAFSHEH, FALAK, REZA, GANJALIKHANI HAKEMI, MAZDAK, & ANDALIB, ALIREZA. (2016). BIOINFORMATIC DESIGNING FOR PRODUCING VACCINE PEPTIDE OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A), AND EVALUATION OF POLYCLONAL ANTIBODIES IN MICE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 34(398), 1054-1059. SID. https://sid.ir/paper/49611/en

Vancouver: Copy

SOLTANPOUR GHARIBDOUSTI FAEZEH, KARDAR GHOLAM ALI, FAZELI DELSHAD BANAFSHEH, FALAK REZA, GANJALIKHANI HAKEMI MAZDAK, ANDALIB ALIREZA. BIOINFORMATIC DESIGNING FOR PRODUCING VACCINE PEPTIDE OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A), AND EVALUATION OF POLYCLONAL ANTIBODIES IN MICE. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2016;34(398):1054-1059. Available from: https://sid.ir/paper/49611/en

IEEE: Copy

FAEZEH SOLTANPOUR GHARIBDOUSTI, GHOLAM ALI KARDAR, BANAFSHEH FAZELI DELSHAD, REZA FALAK, MAZDAK GANJALIKHANI HAKEMI, and ALIREZA ANDALIB, “BIOINFORMATIC DESIGNING FOR PRODUCING VACCINE PEPTIDE OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF-A), AND EVALUATION OF POLYCLONAL ANTIBODIES IN MICE,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 34, no. 398, pp. 1054–1059, 2016, [Online]. Available: https://sid.ir/paper/49611/en

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