SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION

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SALEHI FARZAD; JAFARI RASOOL; SHARAFI SEYEDEH MARYAM; YOUSEFI HOSEINALI; PESTEHCHIAN NADER; YOUSEFI DARANI HOSEIN

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2016 | Volume:34 | Issue:384 Page(s): 589-593

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Title

SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION

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Keywords

CRYPTOSPORIDIUMQ1

MODIFIED-ZIEHL-NEELSENQ1

DOT BLOTQ1

ANTIGEN DETECTIONQ1

SENSITIVITYQ3

SPECIFICITYQ3

Abstract

Background: Diagnosis of cryptosporidiosis is based on the use of the routine microscopic methods, but these methods possess poor SENSITIVITY and also need an expert technician. The aim of the present study was to determine SENSITIVITY and SPECIFICITY of DOT BLOT method for diagnosis of bovine cryptosporidiosis.Methods: In this study 131 fecal samples were collected from suckling calves during summer 2014. The collected samples were concentrated using formalin ether method. The samples were then examined by MODIFIED-ZIEHL-NEELSEN (mZN) and finally all samples were examined by DOT BLOT methods.Findings: Oocysts of CRYPTOSPORIDIUM spp. were found in 40 (30.5%) out of 131 fecal samples using mZN staining method but 49 (37.4%) of the samples were positive for CRYPTOSPORIDIUM antigen using DOT BLOT method.No significant differences were observed among the results of the two methods and the SENSITIVITY and SPECIFICITY of DOT BLOT calculated as 87% and 86%, respectively.Conclusion: Based on the results of the present study, DOT BLOT method showed rather equal performance as MODIFIED-ZIEHL-NEELSEN method.

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APA: Copy

SALEHI, FARZAD, JAFARI, RASOOL, SHARAFI, SEYEDEH MARYAM, YOUSEFI, HOSEINALI, PESTEHCHIAN, NADER, & YOUSEFI DARANI, HOSEIN. (2016). SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 34(384), 589-593. SID. https://sid.ir/paper/50290/en

Vancouver: Copy

SALEHI FARZAD, JAFARI RASOOL, SHARAFI SEYEDEH MARYAM, YOUSEFI HOSEINALI, PESTEHCHIAN NADER, YOUSEFI DARANI HOSEIN. SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2016;34(384):589-593. Available from: https://sid.ir/paper/50290/en

IEEE: Copy

FARZAD SALEHI, RASOOL JAFARI, SEYEDEH MARYAM SHARAFI, HOSEINALI YOUSEFI, NADER PESTEHCHIAN, and HOSEIN YOUSEFI DARANI, “SENSITIVITY AND SPECIFICITY OF DOT BLOT METHOD FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 34, no. 384, pp. 589–593, 2016, [Online]. Available: https://sid.ir/paper/50290/en

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