SEPARATION OF LIPOPROTEIN (A) SUBSPECIES USING LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY

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KADKHODAEI ELYADERANI M.; FAEZIZADEH Z.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Isfahan Medical School Year:2005 | Volume:23 | Issue:79 Page(s): 15-20

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Title

SEPARATION OF LIPOPROTEIN (A) SUBSPECIES USING LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY

Author(s)

KADKHODAEI ELYADERANI M. | FAEZIZADEH Z. | Issue Writer Certificate

Keywords

LIPOPROTEIN (A)Q4

AFFINITY CHROMATOGRAPHYQ3

LYSINE SEPHAROSEQ4

LYSINE BINDING SITEQ4

Abstract

Introduction: Lipoprotein (a) [Lp (a)] is one of the cholesterol-rich plasma lipoproteins, which is a low-density lipoprotein-like particle. Lp (a) displays strong athero-thrombotic properties. In Lp (a), apoprotein (a) [Apo (a)] is covalently linked by a single disulfide bridge to apoproteinB100 (apoB100). Apo (a) is a glycosylated protein similar to plasminogen. Plasminogen is a fibrinolytic proenzyme with kringle-like sequences. Lipoprotein (a) in human plasma appears as four subspecies based on its affinity for LYSINE SEPHAROSE: Lp (a) Lys-, Lp (a) Lys+1, Lp (a) Lys+2 and Lp (a) Lys+3. Pathogenesis of Lp (a) as a risk factor for cardiovascular disease may depend on its lysine-binding site activity. It is suggested that Lp (a) LYS+1, Lp (a) Lys+2 and Lp (a) Lys+3 have, athero-thrombotic properties. In Iran there have not been many studiesori Lp (a) subspecies. Therefore the aim of this study was to separate Lp (a) subspecies according to their affinity for lysine, using LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY. Methods: We separated four different subspecies of Lp (a) by changing pH and molarity of the mobile phase. 100 ml of pooled serum was collected.Results: The total Lp (a) was 46 mg and composed of 12 mg (24%) Lp (a) Lys-, 4 mg (8%), Lp (a) LYS+1, 23 mg (50%), Lp (a) Lys+2, and 4 mg (8%) Lp (a) Lys+3.Electrophoretic mobility of four different subspecies of Lp (a) was assessed on polyacrylamide disk electrophoresis. Purity of the separated fraction was confirmed and there was no difference in electrophoretic mobility between the four subspecies of Lp (a).Discussion: The ability to separate Lp (a) subspecies will enable us to investigate factors affecting atherosclerosis and cardiovascular diseases by studying their effect on Lp (a) subspecies in the future.

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APA: Copy

KADKHODAEI ELYADERANI, M., & FAEZIZADEH, Z.. (2005). SEPARATION OF LIPOPROTEIN (A) SUBSPECIES USING LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 23(79), 15-20. SID. https://sid.ir/paper/50913/en

Vancouver: Copy

KADKHODAEI ELYADERANI M., FAEZIZADEH Z.. SEPARATION OF LIPOPROTEIN (A) SUBSPECIES USING LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2005;23(79):15-20. Available from: https://sid.ir/paper/50913/en

IEEE: Copy

M. KADKHODAEI ELYADERANI, and Z. FAEZIZADEH, “SEPARATION OF LIPOPROTEIN (A) SUBSPECIES USING LYSINE SEPHAROSE AFFINITY CHROMATOGRAPHY,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 23, no. 79, pp. 15–20, 2005, [Online]. Available: https://sid.ir/paper/50913/en

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