CLONING OF THE GENE ENCODING THE P36/LACK PROTEIN FROM LEISHMANIA MAJOR

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HEJAZI S.H.A.D.; FOULADVAND M.A.; MIRMOHAMMAD SADEGHI HAMID; KAZEMI B.; SALEHI MANSOUR

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2005 | Volume:23 | Issue:76 Page(s): 13-19

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Title

CLONING OF THE GENE ENCODING THE P36/LACK PROTEIN FROM LEISHMANIA MAJOR

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Keywords

LEISHMANIA MAJORQ2

LACKQ4

PTZ57RQ4

Abstract

Introduction: LEISHMANIA MAJOR is the causative agent of zoonotic cutaneous leishmaniasis (ZCL), affecting millions of people worldwide. About ten percent of the world's population is at risk and two million new cases are reported annually. This disease is endemic in some parts of Iran and is also a major health problem in this country. LACK protein "among the vaccine candidate proteins of Leishmania" has considerable importance because of its role in regulating the immune responses against this disease.Materials and methods: In this study and for the first time, the LACK gene of the Iranian strain of LEISHMANIA MAJOR was cloned and sequenced. A pair of primers was designed based on the LACK gene sequence in the gene bank, then the LACK gene of LEISHMANIA MAJOR was PCR amplified using the Taq polymerase enzyme. The amplified LACK gene was ligated into the PTZ57R plasmid and transformed into the E. coli Xllblue. The transformed clone was cultured under the appropriate conditions, and the obtained colonies were screened using the different restriction enzymes. After verification by restriction digestion, one of the recombinant plasmids was sequenced using the automated DNA sequencing method.Results: Comparison between the LACK gene sequence from the Iranian strain of LEISHMANIA MAJOR and the LACK gene of LEISHMANIA MAJOR in the gene bank (Accession number U27568) showed a %99 homology. The amplified LACK sequence contained 939 bp, hence encoding a protein encompassing 313 amino acids.Discussion: Complete homology among the LACK gene sequence of the Iranian strain of LEISHMANIA MAJOR and the LACK gene sequence of other LEISHMANIA MAJOR strains and Leishmania genera is in agreement with other studies which have indicated that the LACK gene sequence is conserved among different Leishmania genera. Therefore it can be concluded that the results from the LACK gene of different Leishmania genera can be extended to each other. It is feasible to cut out the LACK sequence from PTZ57R-LACK clone and sub clone it into an appropriate plasmid for the expression and production of the LACK protein. Additionally it would be possible to produce mutations or knocking out a part of this gene to modify the pathogenesis of Leishmania.

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APA: Copy

HEJAZI, S.H.A.D., FOULADVAND, M.A., MIRMOHAMMAD SADEGHI, HAMID, KAZEMI, B., & SALEHI, MANSOUR. (2005). CLONING OF THE GENE ENCODING THE P36/LACK PROTEIN FROM LEISHMANIA MAJOR. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 23(76), 13-19. SID. https://sid.ir/paper/51102/en

Vancouver: Copy

HEJAZI S.H.A.D., FOULADVAND M.A., MIRMOHAMMAD SADEGHI HAMID, KAZEMI B., SALEHI MANSOUR. CLONING OF THE GENE ENCODING THE P36/LACK PROTEIN FROM LEISHMANIA MAJOR. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2005;23(76):13-19. Available from: https://sid.ir/paper/51102/en

IEEE: Copy

S.H.A.D. HEJAZI, M.A. FOULADVAND, HAMID MIRMOHAMMAD SADEGHI, B. KAZEMI, and MANSOUR SALEHI, “CLONING OF THE GENE ENCODING THE P36/LACK PROTEIN FROM LEISHMANIA MAJOR,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 23, no. 76, pp. 13–19, 2005, [Online]. Available: https://sid.ir/paper/51102/en

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