MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA-P) AND LIPOPOLYSACHARIDE EXTRACTED FROM E.COLI ON CULTURED LYMPHOCYTE OF THE CASPIAN SEA SHIP STURGEON (ACIPENSER NUDIVENTRIS)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NOUROUZ FASHKHAMI M.R.; SAFAEIAN SH.; BAHMANI MAHMOUD; CHOUBIAN F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Scientific Fisheries Journal Year:2009 | Volume:18 | Issue:3 Page(s): 157-162

Download Full-Text

Persian Verion

View:

1,468

Download:

Cites:

Information Journal Paper

Title

MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA-P) AND LIPOPOLYSACHARIDE EXTRACTED FROM E.COLI ON CULTURED LYMPHOCYTE OF THE CASPIAN SEA SHIP STURGEON (ACIPENSER NUDIVENTRIS)

Author(s)

NOUROUZ FASHKHAMI M.R. | SAFAEIAN SH. | BAHMANI MAHMOUD | CHOUBIAN F. | Issue Writer Certificate

Keywords

MITOSIS INDEXQ3

SHIP STURGEONQ3

ACIPENSER NUDIVENTRISQ3

PHA-PQ3

LPSQ3

Abstract

The present study was designed to obtain the most effective dose for phtytohemagglutinin (pHA-P) and lipopolysaccharide (LPS) extracted from E. coli to optimize the lymphocyte culture method, the highest mitotic index (MI) and metaphase plates number of SHIP STURGEON, ACIPENSER NUDIVENTRIS. Twenty specimens of two-year old A. nudiventris weighing on average 137g and with an average length of 32cm were used in this study. Different doses (0, 40, 60 and 80 mgmrl) of PHA-P, LPS and a combination of the most effective dose of both mitogenic factors {(40 mgmr -1) PHA-P + (80 mgmr -1) LPS} were added to the culture media and mitotic indices were calculated for each treatment.Results indicated that a dose of 40 mg mr-1 PHA-P and 80 mg mr-1 LPS produced the highest MI (6.26 and 3.02 respectively). Using higher concentrations of PHA-P and LPS resulted in decreased MI, whereas at lower doses of these mitogenic factors, mitotic arrest of cultured lymphocytes was observed. Using a combination of the most effective dose of the two mentioned mitogens {(40 mgmr -1) PHA-P + (80 mgmr -1) LPS} yielded a MI of about 3.84. A dose of 40 mg mr-1 PHA-P produced the highest MI (6.26), the highest number of lymphocytes was cultured and the largest number of metaphase plate was counted.

Cites

No record.

References

No record.

Cite

APA: Copy

NOUROUZ FASHKHAMI, M.R., SAFAEIAN, SH., BAHMANI, MAHMOUD, & CHOUBIAN, F.. (2009). MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA-P) AND LIPOPOLYSACHARIDE EXTRACTED FROM E.COLI ON CULTURED LYMPHOCYTE OF THE CASPIAN SEA SHIP STURGEON (ACIPENSER NUDIVENTRIS). IRANIAN SCIENTIFIC FISHERIES JOURNAL, 18(3), 157-162. SID. https://sid.ir/paper/5149/en

Vancouver: Copy

NOUROUZ FASHKHAMI M.R., SAFAEIAN SH., BAHMANI MAHMOUD, CHOUBIAN F.. MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA-P) AND LIPOPOLYSACHARIDE EXTRACTED FROM E.COLI ON CULTURED LYMPHOCYTE OF THE CASPIAN SEA SHIP STURGEON (ACIPENSER NUDIVENTRIS). IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2009;18(3):157-162. Available from: https://sid.ir/paper/5149/en

IEEE: Copy

M.R. NOUROUZ FASHKHAMI, SH. SAFAEIAN, MAHMOUD BAHMANI, and F. CHOUBIAN, “MITOGENIC EFFECTS OF PHYTOHEMAGGLUTININ (PHA-P) AND LIPOPOLYSACHARIDE EXTRACTED FROM E.COLI ON CULTURED LYMPHOCYTE OF THE CASPIAN SEA SHIP STURGEON (ACIPENSER NUDIVENTRIS),” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 18, no. 3, pp. 157–162, 2009, [Online]. Available: https://sid.ir/paper/5149/en

Related Journal Papers