The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum)

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Hadifar M.; HAGHIGHI KARSIDANI S.; GHASEMI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian Scientific Fisheries Journal Year:2019 | Volume:28 | Issue:2 Page(s): 95-104

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Title

The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum)

Author(s)

Hadifar M. | HAGHIGHI KARSIDANI S. | GHASEMI M. | Issue Writer Certificate

Keywords

Tissue cultureQ3

Caudal finQ1

Rutilus frisii kutumQ1

Abstract

In the present study, the primary cell lines were derived from Caudal fin explants of the Caspian Kutum (Rutilus frisii kutum), using two treatments (tissue culturing without enzyme and after trypsin treatment). For this purpose, pieces of Caudal fin tissue (1 millimeter cubic) were isolated from 10 healthy young fish with an average weight of 12± 1 g and a mean length of 11± 1cm in sterile condition then were explanted in L-15 medium (Leibovitz L-15) with fetal bovine serum (10 and 20%) and penicillin-streptomycyne at pH 6. 8 and 7. 2 and kept at 21 and 24 ° C. From the second day, a mixture of various cell types was observed and attached to the cell culture flask, but the uniform cell layer consist of cells with heterogeneous epithelial morphology and fibroblast-like were appeared after 12 to 14 days. Subculture was performed using trypsin enzyme. In subcultures, a single cell layer consists of cells with homogeneous epithelial morphology was formed. So far, these cells have been passaged for 21 times. Results indicate the possibility of producing a permanent cell line from the fin tissue of Rutilus frisii kutum.

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APA: Copy

Hadifar, M., HAGHIGHI KARSIDANI, S., & GHASEMI, M.. (2019). The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum). IRANIAN SCIENTIFIC FISHERIES JOURNAL, 28(2 ), 95-104. SID. https://sid.ir/paper/5173/en

Vancouver: Copy

Hadifar M., HAGHIGHI KARSIDANI S., GHASEMI M.. The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum). IRANIAN SCIENTIFIC FISHERIES JOURNAL[Internet]. 2019;28(2 ):95-104. Available from: https://sid.ir/paper/5173/en

IEEE: Copy

M. Hadifar, S. HAGHIGHI KARSIDANI, and M. GHASEMI, “The Primary cell culture from caudal fin tissue of Caspian Kutum (Rutilus frisii kutum),” IRANIAN SCIENTIFIC FISHERIES JOURNAL, vol. 28, no. 2 , pp. 95–104, 2019, [Online]. Available: https://sid.ir/paper/5173/en

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