Genotyping of Escherichia coli strains isolated from urinary tract infections in Chaharmahal and Bakhtiari province

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MOMTAZ HASSAN; Banisharif Dehkordi Gholamreza; Banisharif Dehkordi Fatemeh; Banitalebi Dehkordi Shokoofeh

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Journal: MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:61 | Issue:2 Page(s): 940-949

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Title

Genotyping of Escherichia coli strains isolated from urinary tract infections in Chaharmahal and Bakhtiari province

Author(s)

MOMTAZ HASSAN | Banisharif Dehkordi Gholamreza | Banisharif Dehkordi Fatemeh | Banitalebi Dehkordi Shokoofeh | Issue Writer Certificate

Keywords

Uropathogenic Escherichia coli (UPEC)Q1

genetic classificationQ1

ERIC-PCRQ1

Chahrmahal & Bakhtiari provinceQ2

Abstract

Background & objectives: Uropathogenic Escherichia coli (UPEC) is one of the most prevalent microbial factor resulting in urinary system infections. Many of its serotypes with genetic variety contribute to these infections. The present study was done aiming at genetic classification of UPEC strains isolated from urinary system infections in Chahrmahal & Bakhtiari province, Iran. Materials & Methods: A total of 67 isolates of UPEC from patients hospitalized in hospitals of Chahrmahal & Bakhtiari were chosen and tested in ERIC-PCR. Results: The studied isolates had band pattern varying from 110-3100 bp which were classified to 15 subgroups in the resulting banding pattern with simple matching similarity coefficient in 91% similarity level. Except 100 proximity found in four cases, other isolates had 44. 4-48. 1% genetic proximity. Placement of the studied isolates in several subgroups showed the acceptable discrimination power of ERIC-PCR technique in genotyping/ E. coli and presence of various sources of contamination of urinary system with this pathogen. Conclusion: ERIC-PCR is a simple, fast and low cost method for describing the genetic variety of different strains of E. coli including UPEC strains.

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APA: Copy

MOMTAZ, HASSAN, Banisharif Dehkordi, Gholamreza, Banisharif Dehkordi, Fatemeh, & Banitalebi Dehkordi, Shokoofeh. (2018). Genotyping of Escherichia coli strains isolated from urinary tract infections in Chaharmahal and Bakhtiari province. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, 61(2 ), 940-949. SID. https://sid.ir/paper/51970/en

Vancouver: Copy

MOMTAZ HASSAN, Banisharif Dehkordi Gholamreza, Banisharif Dehkordi Fatemeh, Banitalebi Dehkordi Shokoofeh. Genotyping of Escherichia coli strains isolated from urinary tract infections in Chaharmahal and Bakhtiari province. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;61(2 ):940-949. Available from: https://sid.ir/paper/51970/en

IEEE: Copy

HASSAN MOMTAZ, Gholamreza Banisharif Dehkordi, Fatemeh Banisharif Dehkordi, and Shokoofeh Banitalebi Dehkordi, “Genotyping of Escherichia coli strains isolated from urinary tract infections in Chaharmahal and Bakhtiari province,” MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, vol. 61, no. 2 , pp. 940–949, 2018, [Online]. Available: https://sid.ir/paper/51970/en

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