CLONING OF PARTIALLY DIGESTED FRAGMENTS OF BACILLUS CEREUS CHROMOSOMAL DNA IN BACTERIOPHAGE LAMBDA, CONSTRUCTION OF A AZAPDNALIBRARY

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BEHRAVAN J.; MOIR ANNE

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Journal: Iranian Journal of Basic Medical Sciences Year:2002 | Volume:4 | Issue:4 (12) Page(s): 201-206

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Title

CLONING OF PARTIALLY DIGESTED FRAGMENTS OF BACILLUS CEREUS CHROMOSOMAL DNA IN BACTERIOPHAGE LAMBDA, CONSTRUCTION OF A AZAPDNALIBRARY

Author(s)

BEHRAVAN J. | MOIR ANNE | Issue Writer Certificate

Keywords

LANDA ZAPLIBRARYQ4

B.CEREUSQ2

CLONINGQ2

Abstract

A λZAP library of B. cereus 569 UM20.1 chromosomal DNA was constructed and screened for the desired clone. Chromosomal DNA from the above strain was extracted and partially digested with Sau3A restriction enzyme. DNA fragments with 4-9 kb size were cut out of the agarose gel and purified. The DNA was then cloned into BamHI digested Lambda arms and the reaction was packaged. In order to determine the concentration of infective phage particles and the proportion containing insert DNA, the packaged extract was titered and a recovery of 2.8×105 pfu.µg-1 of vector was obtained. In vivo excision of the pBK-CMV plasmid from the λZAP express vector was carried out and resulted in isolation of two plasmids,pJB1 and pJB2. The two plasmids were used for DNA sequence analysis.

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APA: Copy

BEHRAVAN, J., & MOIR, ANNE. (2002). CLONING OF PARTIALLY DIGESTED FRAGMENTS OF BACILLUS CEREUS CHROMOSOMAL DNA IN BACTERIOPHAGE LAMBDA, CONSTRUCTION OF A AZAPDNALIBRARY. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 4(4 (12)), 201-206. SID. https://sid.ir/paper/52368/en

Vancouver: Copy

BEHRAVAN J., MOIR ANNE. CLONING OF PARTIALLY DIGESTED FRAGMENTS OF BACILLUS CEREUS CHROMOSOMAL DNA IN BACTERIOPHAGE LAMBDA, CONSTRUCTION OF A AZAPDNALIBRARY. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2002;4(4 (12)):201-206. Available from: https://sid.ir/paper/52368/en

IEEE: Copy

J. BEHRAVAN, and ANNE MOIR, “CLONING OF PARTIALLY DIGESTED FRAGMENTS OF BACILLUS CEREUS CHROMOSOMAL DNA IN BACTERIOPHAGE LAMBDA, CONSTRUCTION OF A AZAPDNALIBRARY,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 4, no. 4 (12), pp. 201–206, 2002, [Online]. Available: https://sid.ir/paper/52368/en

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