INCREASING THE IMMUNE RESPONSES BY CO-DELIVERY OF CPG-ODN AND TETANUS TOXOID ENCAPSULATED IN PLGA MICROSPHERES

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SAJADI TABASSI S.A.; JAFARI M.R.; TAFAGHODI M.; DIVAN M.; SAMUEL J.

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Journal: Iranian Journal of Basic Medical Sciences Year:2003 | Volume:6 | Issue:3 (19) Page(s): 221-236

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Title

INCREASING THE IMMUNE RESPONSES BY CO-DELIVERY OF CPG-ODN AND TETANUS TOXOID ENCAPSULATED IN PLGA MICROSPHERES

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Keywords

TETANUS TOXOIDQ4

CPGQ4

OLIGODEOXYNUCLEOTIDESQ4

MICROSPHERESQ3

ALUMQ2

ADJUVANTQ2

Abstract

Oligodeoxynucleotides (ODN) having a CPG motif (CpG-ODN) are regarded as a new class of potent immunoadjuvants. It has been shown that concomitant delivery of an antigen with CPG-ODN to the same APC will be able to increase the immune responses against the relevant antigen. The main objective of the present study was co-encapsulation of TETANUS TOXOID (TT) and CPG-ODN in PLGA MICROSPHERES and evaluation of immune responses gained by the concomitant delivery of TT and CPG-ODN via the same delivery system. Both TT alone and [TT+CpG-ODN] were encapsulated in PLGA MICROSPHERES. The following groups of mice were immunized twice 2 Lf TT in the presence or absence of 4µg CPG-ODN via subcutaneous route: 1) encapsulated [TT + CPG-ODN]; 2) encapsulated TT alone; 3) encapsulated TT with CPG-ODN solution; 4) solution of [TT+ CPG-ODN]; 5) ALUM adsorbed TT (routine vaccine). Mice immunized with MICROSPHERES encapsulated with [TT+CpG-ODN] showed a very high T cell proliferation (stimulation index=45 in T cell proliferation assay and very high levels of INF-γ (2694.7±41.1 pg/ml) which was significantly higher than those of other groups. Anti-sera obtained from this group also showed very high total IgG titers (end point titers 2560000) which was 16 times that of the group immunized with the solution of [TT+CpG-ODN]. Also the titers of IgGl, IgG3 and IgG2b obtained from group 1 were considerably higher than those from other groups. Interestingly however the IgG titers induced in group 5 were far less than that in group 1 (total IgG, end point titers 160000). This study clearly showed that [TT+CpG-ODN] encapsulated in PLGA MICROSPHERES was more efficient than the relevant solution in terms of inducing immune responses. Also the results indicated that the co-delivery of TT and CPG-ODN in PLGA MICROSPHERES elicits a stronger Th1 (i.e. IgG2b, IgG3, INF- γ ) as well as Th2 (i.e. IgG1) type responses.

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APA: Copy

SAJADI TABASSI, S.A., JAFARI, M.R., TAFAGHODI, M., DIVAN, M., & SAMUEL, J.. (2003). INCREASING THE IMMUNE RESPONSES BY CO-DELIVERY OF CPG-ODN AND TETANUS TOXOID ENCAPSULATED IN PLGA MICROSPHERES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 6(3 (19)), 221-236. SID. https://sid.ir/paper/52384/en

Vancouver: Copy

SAJADI TABASSI S.A., JAFARI M.R., TAFAGHODI M., DIVAN M., SAMUEL J.. INCREASING THE IMMUNE RESPONSES BY CO-DELIVERY OF CPG-ODN AND TETANUS TOXOID ENCAPSULATED IN PLGA MICROSPHERES. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2003;6(3 (19)):221-236. Available from: https://sid.ir/paper/52384/en

IEEE: Copy

S.A. SAJADI TABASSI, M.R. JAFARI, M. TAFAGHODI, M. DIVAN, and J. SAMUEL, “INCREASING THE IMMUNE RESPONSES BY CO-DELIVERY OF CPG-ODN AND TETANUS TOXOID ENCAPSULATED IN PLGA MICROSPHERES,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 6, no. 3 (19), pp. 221–236, 2003, [Online]. Available: https://sid.ir/paper/52384/en

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