DETECTION OF MINIMAL RESIDUAL DISEASE IN CML PATIENT AFTER BONE MARROW TRANSPLANTATION BY RT-PCR

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ABBASZADEGAN M.R.; SHIRDEL A.; SOROURI J.

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Journal: Iranian Journal of Basic Medical Sciences Year:2001 | Volume:4 | Issue:3 (11) Page(s): 147-152

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Title

DETECTION OF MINIMAL RESIDUAL DISEASE IN CML PATIENT AFTER BONE MARROW TRANSPLANTATION BY RT-PCR

Author(s)

Keywords

CMLQ3

BCR/ABLQ4

MRDQ4

RT-PCRQ3

Abstract

Philadelphia chromosome can be founding 95% of patients with chronic myeloid leukemia (CML) by cytogenetic studies. The fused BCR/ABL is transcribed in two types of chimeric mRNA. RT-PCR amplification of these two transcripts have been designed to give two different size products. This assay can detect one positive BCR/ABL expressing cell in a back ground of 106 negative BCR/ABL cells. The power of this assay is the detection of minimal residual disease (MRD) between 6-12 months following bone marrow transplantation (BMT) is an independent and significant factor that predicts the relapse in future. The aim of optimization was to detect β2 microglobulin (β 2M) mRNA. Detection of β2 mRNA and absence of BCR/ABL indicates that the patient is negative for MRD and as a result, there is molecular remission in addition to clinical remission. To monitor MRD we tested a patient's blood sample who had tolerated allogenic BMT 7 years ago. BCR/ABL wasn't detected in this patient and only β2 M was observed. This result confirmed the absence of MRD in this case. To effectively monitor minimal leukemic activity after BMT, we used a competitive RT- PCR to quantify expression of the characteristic BCR/ABL fusion gene mRNA in patients with CML.

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APA: Copy

ABBASZADEGAN, M.R., SHIRDEL, A., & SOROURI, J.. (2001). DETECTION OF MINIMAL RESIDUAL DISEASE IN CML PATIENT AFTER BONE MARROW TRANSPLANTATION BY RT-PCR. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 4(3 (11)), 147-152. SID. https://sid.ir/paper/52459/en

Vancouver: Copy

ABBASZADEGAN M.R., SHIRDEL A., SOROURI J.. DETECTION OF MINIMAL RESIDUAL DISEASE IN CML PATIENT AFTER BONE MARROW TRANSPLANTATION BY RT-PCR. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2001;4(3 (11)):147-152. Available from: https://sid.ir/paper/52459/en

IEEE: Copy

M.R. ABBASZADEGAN, A. SHIRDEL, and J. SOROURI, “DETECTION OF MINIMAL RESIDUAL DISEASE IN CML PATIENT AFTER BONE MARROW TRANSPLANTATION BY RT-PCR,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 4, no. 3 (11), pp. 147–152, 2001, [Online]. Available: https://sid.ir/paper/52459/en

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