PHENOTYPIC AND MOLECULAR EVALUATION OF ESBL PRODUCTION AMONG E.COLI STRAINS ISOLATED FROM ORTHOPEDIC WOUNDS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KAYVANI H.; HAKEMI VALA M.; TALEBNIA CHALANBAR Z.; MIRBAGHERI F.; BAGHERI BEJESTANI F.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2013 | Volume:17 | Issue:59 Page(s): 29-31

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

766

Download:

Cites:

Information Journal Paper

Title

PHENOTYPIC AND MOLECULAR EVALUATION OF ESBL PRODUCTION AMONG E.COLI STRAINS ISOLATED FROM ORTHOPEDIC WOUNDS

Author(s)

Keywords

E.COLIQ3

PER-1Q2

ESBLQ2

Abstract

Background and objective: Escherichia coli strains are a member of Enterobacteriacea which are commonly isolated from orthopedic wounds. Recently, by ESBL production, E.COLI strains are resistant to most common antibiotics and causes strong worldwide problem in treatment. The aim of this study was evaluation of ESBL production among E.COLI strains isolated from orthopedic wounds of Firozgar and Akhtar hospitals during 1389-90 by phenotypic and molecular methods.Materials and methods: During 1389-90 (2011), 60 orthopedic wounds were collected from Firozgar and Akhtar hospitals by sterile condition. The E.COLI strains were isolated and identified based on bacterial and biochemical standard methods. Their resistant evaluated against common antibiotics by disk diffusion method (CLSI standard). ESBL screening was done by phenotypic DDST/DDT methods. Consequently, DNA was extracted by kit and the frequency of TEM, SHV, PER-1 genes were evaluated by PCR method.Results: Of 60 collected orthopedic wounds from Firozgar and akhtar hospitals, E.COLI strains (48.33%) was detected. Their resistant to tested antibiotics by Kirby-Bauer method were, 41.73% to cefpodoxim, 68.96% to cefotaxim, 51.72% to ceftazidim, 48.27% to cephalexin and gentamicin, ciprofloxacin, chloramphenicle, cefepime and imipenem 44.82%, ceftriaxone 41.37% and aztronam 34.48%. By DDST/DDT methods, 20.68% of E.COLI strains were ESBL positive. The frequency of SHV, TEM and PER-1 were 33%, 33% and 50%, respectively by PCR method.Conclusion: Based on searches, ESBL production in E.COLI strains is mostly related to CTXM, SHV, TEM genes and PER-1 was detected before in Pseudomonas spp, Acinetobacter spp, Proteus spp. The co-existence of PER-1 was reported in 7.5% of Klebsiella strains in Iran. Based on data, this is the first time that PER-1 was detected in E.COLI strains without existence of any other ESBL genes. However, the confirmation of this results need more sample size, sequencing and comparison the results by data bank.

Cites

No record.

References

No record.

Cite

APA: Copy

KAYVANI, H., HAKEMI VALA, M., TALEBNIA CHALANBAR, Z., MIRBAGHERI, F., & BAGHERI BEJESTANI, F.. (2013). PHENOTYPIC AND MOLECULAR EVALUATION OF ESBL PRODUCTION AMONG E.COLI STRAINS ISOLATED FROM ORTHOPEDIC WOUNDS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 17(59), 29-31. SID. https://sid.ir/paper/52761/en

Vancouver: Copy

KAYVANI H., HAKEMI VALA M., TALEBNIA CHALANBAR Z., MIRBAGHERI F., BAGHERI BEJESTANI F.. PHENOTYPIC AND MOLECULAR EVALUATION OF ESBL PRODUCTION AMONG E.COLI STRAINS ISOLATED FROM ORTHOPEDIC WOUNDS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2013;17(59):29-31. Available from: https://sid.ir/paper/52761/en

IEEE: Copy

H. KAYVANI, M. HAKEMI VALA, Z. TALEBNIA CHALANBAR, F. MIRBAGHERI, and F. BAGHERI BEJESTANI, “PHENOTYPIC AND MOLECULAR EVALUATION OF ESBL PRODUCTION AMONG E.COLI STRAINS ISOLATED FROM ORTHOPEDIC WOUNDS,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 17, no. 59, pp. 29–31, 2013, [Online]. Available: https://sid.ir/paper/52761/en

Related Journal Papers