GENERATION MUTANT CONSTRUCT OF PGEM-7ZF::ΔICSA (CATR) FOR ATTENUATION OF SHIGELLA FLEXNERI 2A

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DOROUDIAN M.; SAADATI M.; GHAHROUDI A.; JAHANTIGH D.; SALEHI A.R.; HOSSEINI M.; EFTEKHARI M.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2013 | Volume:17 | Issue:59 Page(s): 41-47

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Title

GENERATION MUTANT CONSTRUCT OF PGEM-7ZF::ΔICSA (CATR) FOR ATTENUATION OF SHIGELLA FLEXNERI 2A

Author(s)

Keywords

SHIGELLA FLEXNERI2AQ2

ICSA GENEQ2

ALLELIC EXCHANGEQ2

LIVE ATTENUATED VACCINEQ2

Abstract

Background and objective: Shigellosis causes the most contagious diseases, acute diarrheal disease in the world. The ICSA GENE plays a key role in pathogenesis in bacterial. The aim of this study was cloning of ICSA GENE and developing a mutant constructpGEM:: DicsA (Catr) in order to induction recombination in a native shigella for generation a LIVE ATTENUATED VACCINE candidate strain.Materials and methods: by use of biochemical tests, monoclonal Antibody and PCR the native shigella strain was examined. The entire ICSA GENE specific primers were designed, the DicsA gene was cloned in pGEM-7zf vector and the nucleotide sequence was determined. According to the data of sequencing, digestion mapping of pGEM DvirG was obtained, after digested of DicsA gene by EcoRV restriction enzyme a chloramphenicol antibiotic resistance (catr) gene Cloned in amidst of DicsA gene.Results: The native shigella strain by using biochemical tests was confirmed. Sequence of the ICSA GENE in native strain was sequentially identical with the strains submitted in the Gene-Bank database.Structure accuracy using specific primerspGEM:: DicsA (Catr) was confirmed.Conclusion: Using the technique of ALLELIC EXCHANGE based on the incident of recombination in bacteria is one of the most effective methods to develop a disruption in the target genes. This mutant construct can be applied in development of a live attenuated SHIGELLA FLEXNERI2A vaccine candidate.

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APA: Copy

DOROUDIAN, M., SAADATI, M., GHAHROUDI, A., JAHANTIGH, D., SALEHI, A.R., HOSSEINI, M., & EFTEKHARI, M.. (2013). GENERATION MUTANT CONSTRUCT OF PGEM-7ZF::ΔICSA (CATR) FOR ATTENUATION OF SHIGELLA FLEXNERI 2A. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 17(59), 41-47. SID. https://sid.ir/paper/52763/en

Vancouver: Copy

DOROUDIAN M., SAADATI M., GHAHROUDI A., JAHANTIGH D., SALEHI A.R., HOSSEINI M., EFTEKHARI M.. GENERATION MUTANT CONSTRUCT OF PGEM-7ZF::ΔICSA (CATR) FOR ATTENUATION OF SHIGELLA FLEXNERI 2A. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2013;17(59):41-47. Available from: https://sid.ir/paper/52763/en

IEEE: Copy

M. DOROUDIAN, M. SAADATI, A. GHAHROUDI, D. JAHANTIGH, A.R. SALEHI, M. HOSSEINI, and M. EFTEKHARI, “GENERATION MUTANT CONSTRUCT OF PGEM-7ZF::ΔICSA (CATR) FOR ATTENUATION OF SHIGELLA FLEXNERI 2A,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 17, no. 59, pp. 41–47, 2013, [Online]. Available: https://sid.ir/paper/52763/en

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