DETECTION OF MULTI-DRUG RESISTANCE GENES IN UROPATHOGENIC E. COLI BY MULTIPLEX-PCR

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SIASI ELHAM; RAFIEI TABATABAII ROBAB; YASERI ELHAM

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2017 | Volume:21 | Issue:75 Page(s): 43-52

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Title

DETECTION OF MULTI-DRUG RESISTANCE GENES IN UROPATHOGENIC E. COLI BY MULTIPLEX-PCR

Author(s)

SIASI ELHAM | RAFIEI TABATABAII ROBAB | YASERI ELHAM | Issue Writer Certificate

Keywords

UPECQ1

(DFRA1Q1

TEMQ1

TETBQ1

CAT1) GENESQ1

MULTIPLEX-PCRQ1

Abstract

Background and objective: Urinary tract infection caused by E. coli is one of the most common infections in the all group age in worldwide. The aim of this study was identified of dfrA1, TEM, tetB and cat1 multi drug resistance genes in the Uropathogenic E.coli isolated from clinical samples by multiplex-PCR.Materials and methods: 100 samples isolated from patients with urinary tract infections. For detection of E.coli was used biochemical and microbiological tests. By antibiogram was identified antibiotic resistance of E.coli strain. Then prevalence of dfrA1, TEM, tetB and cat1genes in the Uropathogenic E.coli isolated was finded by multiplex-PCR.Results: From 100 samples, 63 were E. coli strains. The highest and lowest antibiotic resistance were detected to chloramphenicol (92.1%) and tetracycline (33.3%). By molecular analysis was identified frequency of studied genes, as, dfrA1 (38.1%), TEM (65.1%), cat1 (92.1%) (highest), and tetB (25.4%) (lowest).Conclusion: Prevalence of urinary tract infection in the community by E.coli and distribution of it’s resistance and virulence factors, the fast and perfect detection of this bacteria and it’s resistance genes are necessary.

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APA: Copy

SIASI, ELHAM, RAFIEI TABATABAII, ROBAB, & YASERI, ELHAM. (2017). DETECTION OF MULTI-DRUG RESISTANCE GENES IN UROPATHOGENIC E. COLI BY MULTIPLEX-PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 21(75), 43-52. SID. https://sid.ir/paper/52769/en

Vancouver: Copy

SIASI ELHAM, RAFIEI TABATABAII ROBAB, YASERI ELHAM. DETECTION OF MULTI-DRUG RESISTANCE GENES IN UROPATHOGENIC E. COLI BY MULTIPLEX-PCR. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2017;21(75):43-52. Available from: https://sid.ir/paper/52769/en

IEEE: Copy

ELHAM SIASI, ROBAB RAFIEI TABATABAII, and ELHAM YASERI, “DETECTION OF MULTI-DRUG RESISTANCE GENES IN UROPATHOGENIC E. COLI BY MULTIPLEX-PCR,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 21, no. 75, pp. 43–52, 2017, [Online]. Available: https://sid.ir/paper/52769/en

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