EVALUATION OF THE ELISA- IGG FOR THE DIAGNOSIS OF ACUTE HUMAN BRUCELLOSIS

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RANJBAR M.; KARIMI B.; MONSEF ESFAHANI A.R.; MANI KASHANI KH.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2006 | Volume:11 | Issue:34 Page(s): 35-39

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Title

EVALUATION OF THE ELISA- IGG FOR THE DIAGNOSIS OF ACUTE HUMAN BRUCELLOSIS

Author(s)

RANJBAR M. | KARIMI B. | MONSEF ESFAHANI A.R. | MANI KASHANI KH. | Issue Writer Certificate

Keywords

BRUCELLOSISQ3

DIAGNOSTIC TESTSQ3

ROUTINEQ3

ENZYME-LINKED IMMUNOSORBENT ASSAYQ3

Abstract

Background and objective: BRUCELLOSIS is an important public health problem that occurs worldwide. Its clinical manifestations can be quite varied and definitive signs to indicate the diagnosis can be lacking and the clinical diagnosis must usually supported by the results of bacteriologic and / or serologic tests. According to recent reports in Iran our city "Hamedan" is a one of the cities with high incidence of BRUCELLOSIS (incidence=107.5/100000 per year).Materials & methods: In this diagnostic study, patients with clinical diagnosis of BRUCELLOSIS that had referred to Sina hospital were evaluated. A combination of infection diseases specialist clinical diagnosis and positive Wright test (Wright test ≥ 1/80) or positive 2ME test (2me ≥1/40) defined as a gold standard for diagnosis of BRUCELLOSIS. Control's group was medical students and some of their families that have negative Wright and 2me tests and clinical diagnosis of BRUCELLOSIS.Results: Over a one-year period, we had 200 patients (120 male and 80 female) with clinical features suggestive of BRUCELLOSIS and 200 medical students and some of their families as a control group. The average age in patients was 38.74 years (min=6, max=76, SD=17.3) and in control group was 33.38 years (min=6, max=70, SD=14.5). All the 200 controls had a negative Wright and 2ME test results. The sensitivity and specificity of the ELISA were 92% and 100% respectively. The positive and negative predictive values were 100% and 92.5% respectively.Conclusion: The ELISA is a rapid, reliable, easy to perform and sensitive test in the diagnosis of BRUCELLOSIS. It saves laboratory cost and time.

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APA: Copy

RANJBAR, M., KARIMI, B., MONSEF ESFAHANI, A.R., & MANI KASHANI, KH.. (2006). EVALUATION OF THE ELISA- IGG FOR THE DIAGNOSIS OF ACUTE HUMAN BRUCELLOSIS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 11(34), 35-39. SID. https://sid.ir/paper/52877/en

Vancouver: Copy

RANJBAR M., KARIMI B., MONSEF ESFAHANI A.R., MANI KASHANI KH.. EVALUATION OF THE ELISA- IGG FOR THE DIAGNOSIS OF ACUTE HUMAN BRUCELLOSIS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2006;11(34):35-39. Available from: https://sid.ir/paper/52877/en

IEEE: Copy

M. RANJBAR, B. KARIMI, A.R. MONSEF ESFAHANI, and KH. MANI KASHANI, “EVALUATION OF THE ELISA- IGG FOR THE DIAGNOSIS OF ACUTE HUMAN BRUCELLOSIS,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 11, no. 34, pp. 35–39, 2006, [Online]. Available: https://sid.ir/paper/52877/en

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