NEISSERIA MENINGITIDES SEROGROUP B OUTER MEMBRANE VESICLE AS A MENINGOCOCCUS VACCINE CANDIDATE IN ANIMAL MODEL

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SIADAT D.; TABARAEI BAHMAN; BEHZADIANNEZHAD GH.; NOUROUZIAN D.; AHMADI HOJAT; NAJAR PIRAYEH SH.; MOHABATI MOBAREZ A.; NEJATI M.; HEDAYATEI M.H.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2007 | Volume:12 | Issue:36 Page(s): 11-17

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Title

NEISSERIA MENINGITIDES SEROGROUP B OUTER MEMBRANE VESICLE AS A MENINGOCOCCUS VACCINE CANDIDATE IN ANIMAL MODEL

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Keywords

NEISSERIA MENINGITIDES SEROGROUP BQ3

EXTRACTIONQ3

SERUM BACTERICIDAL ACTIVITYQ3

Abstract

Background and objective: Bacterial meningitis caused by different groups of Neisseria meningitidis is still one of the serious health problems worlds wide. The serum bactericidal assay (SBA) to meningococci is the most important test in immunological evaluation of meningococcal infection after vaccination. The SBA has been adapted as the gold standard for immunity against different serogroups of Neisseria meningitides after immunization.Material and methods: In at present investigation, standard group B strain of Neisseria meningitidis (CSBPI, G-245) were grown under controlled-submerge cultural conditions in fermenter containing 40 liters of modified Frantz medium. The cells were harvested at late exponential growth phase. OMV were extracted from cell wall according to the Deoxycholate EXTRACTION Technique. The pletted OMVs were homogenized in PBS containing sucrose and thiomersal as preservative and further evaluation was performed. OMV and control were injected intramuscularly into groups of five rabbit with boosters on days 14, 28 and 42 after the primary immunization. The serum collected on days 0, 14, 28, 42 and 56 were tested by complement mediated bactericidal assay according to the world health organization protocol.Results: More than 70-90 % of the OMV retained their native configurationally structure with size ranges from 50 to 150 nm after EXTRACTION and purification procedures. This was shown by micrograph scanning of OMV in Zeisse CEM 902-A electron microscope. Purified vesicle showed a strong band of 40-45 KD molecular weights when run on 10% polyacrylamide gel electrophoresis with SDS. Identities of the OMVs were determined by Double Diffusion Gel Technique using hyperimmune rabbit anti OMV-PorA antisera against purified vesicles.The results of SBA with OMV revealed a significant increase in serum bactericidal titer against serogroup B meningococci after 56 day in comparison with the control group.Conclusion: The results indicate that OMV is able to induce a high level of bactericidal antibody response. Therefore, this paper shows that OMV could be a candidate for Vaccine toward serogroup B meningococci.

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APA: Copy

SIADAT, D., TABARAEI, BAHMAN, BEHZADIANNEZHAD, GH., NOUROUZIAN, D., AHMADI, HOJAT, NAJAR PIRAYEH, SH., MOHABATI MOBAREZ, A., NEJATI, M., & HEDAYATEI, M.H.. (2007). NEISSERIA MENINGITIDES SEROGROUP B OUTER MEMBRANE VESICLE AS A MENINGOCOCCUS VACCINE CANDIDATE IN ANIMAL MODEL. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 12(36), 11-17. SID. https://sid.ir/paper/52949/en

Vancouver: Copy

SIADAT D., TABARAEI BAHMAN, BEHZADIANNEZHAD GH., NOUROUZIAN D., AHMADI HOJAT, NAJAR PIRAYEH SH., MOHABATI MOBAREZ A., NEJATI M., HEDAYATEI M.H.. NEISSERIA MENINGITIDES SEROGROUP B OUTER MEMBRANE VESICLE AS A MENINGOCOCCUS VACCINE CANDIDATE IN ANIMAL MODEL. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2007;12(36):11-17. Available from: https://sid.ir/paper/52949/en

IEEE: Copy

D. SIADAT, BAHMAN TABARAEI, GH. BEHZADIANNEZHAD, D. NOUROUZIAN, HOJAT AHMADI, SH. NAJAR PIRAYEH, A. MOHABATI MOBAREZ, M. NEJATI, and M.H. HEDAYATEI, “NEISSERIA MENINGITIDES SEROGROUP B OUTER MEMBRANE VESICLE AS A MENINGOCOCCUS VACCINE CANDIDATE IN ANIMAL MODEL,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 12, no. 36, pp. 11–17, 2007, [Online]. Available: https://sid.ir/paper/52949/en

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