THE EFFECT OF VOLTAGE AND DORATION OF ELECTRICAL
STIMOLOS ON FOSION AND CLEAVAGE OF ELECTROFOSED TWO-CELL
BOVINE EMBRYOS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NASRESFAHANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2005 | Volume:6 | Issue:24 Page(s): 226-231

Download Full-Text

View:

358

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF VOLTAGE AND DORATION OF ELECTRICAL STIMOLOS ON FOSION AND CLEAVAGE OF ELECTROFOSED TWO-CELL BOVINE EMBRYOS

Author(s)

NASRESFAHANI MOHAMMAD HOSSEIN | Issue Writer Certificate

Keywords

BOVINE

ELECTROFUSION

TETRAPLOID

VOLTAGE

Abstract

Introduction: The value of embryonic stem-like cells in cloning is obvious. Production of cloned animals can be achieved by introduction of these cells into a tetraploid embryo. TETRAPLOID embryo is used as a feeder for development of embryonic stem-like cells and can be produced in vitro by electro-fusion of 2-cell embryos. The aim of this study was to ass,essthe effect of VOLTAGE alteration and duration on fusion and cleavage rates of BOVINE TETRAPLOID embryo produced by electrofusion. Material and Methods: After in vitro maturation and fertilization of cumulus oocyte complexes, two-cell embryos were categorized into three groups: 1- Fused group (FG): include two-cell embryos fused by exposure to different VOLTAGEs (0.5, 0.75, 1, 1.25 1.:& kV/cm) and durations (20, 40, 60, 80 & 100 I-Is).2- Exposed control group (ECG): two-cell embryos that remained unfused after ELECTROFUSION. 3- Unexposed control group (UCG): two-cell embryos cultured without exposure to any VOLTAGE. The embryos from each groupWere cultured in SOF1. The fusion and cleavage rates were compared in each group. Results: Increase in VOLTAGE resulted in significantly higher fusion and lower cleavage rate. The increased duration had no significant effect on fusion rate. The increased duration of high VOLTAGEs caused decreased cleavage rate significantly, and in low VOLTAGE resulted in increased cleavage rate. The cleavage rate in ECG group followed the same as FG group, and they were lower when compared to UCG. Conclusion: Best fusion and cleavage !:ate was obtained at 0.75-1 kV/cm for 60l-ls duration.

Cites

No record.

References

No record.

Cite

APA: Copy

NASRESFAHANI, MOHAMMAD HOSSEIN. (2005). THE EFFECT OF VOLTAGE AND DORATION OF ELECTRICAL STIMOLOS ON FOSION AND CLEAVAGE OF ELECTROFOSED TWO-CELL BOVINE EMBRYOS. CELL JOURNAL (YAKHTEH), 6(24), 226-231. SID. https://sid.ir/paper/529582/en

Vancouver: Copy

NASRESFAHANI MOHAMMAD HOSSEIN. THE EFFECT OF VOLTAGE AND DORATION OF ELECTRICAL STIMOLOS ON FOSION AND CLEAVAGE OF ELECTROFOSED TWO-CELL BOVINE EMBRYOS. CELL JOURNAL (YAKHTEH)[Internet]. 2005;6(24):226-231. Available from: https://sid.ir/paper/529582/en

IEEE: Copy

MOHAMMAD HOSSEIN NASRESFAHANI, “THE EFFECT OF VOLTAGE AND DORATION OF ELECTRICAL STIMOLOS ON FOSION AND CLEAVAGE OF ELECTROFOSED TWO-CELL BOVINE EMBRYOS,” CELL JOURNAL (YAKHTEH), vol. 6, no. 24, pp. 226–231, 2005, [Online]. Available: https://sid.ir/paper/529582/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops