EVALUATION OF RESISTANCE TO B-LACTAM ANTIBIOTICS IN STAPHYLOCOCCI USING PENICILLIN AND OXACILLIN DISKS

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DANESH MONFARD S.; EFTEKHAR F.

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2004 | Volume:8 | Issue:23 Page(s): 1-5

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Title

EVALUATION OF RESISTANCE TO B-LACTAM ANTIBIOTICS IN STAPHYLOCOCCI USING PENICILLIN AND OXACILLIN DISKS

Author(s)

DANESH MONFARD S. | EFTEKHAR F. | Issue Writer Certificate

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Abstract

Staphylococci are the most common pathogens in hospital and community acquired infections. Beta lactam drugs, especially third generation cephalosporin's have been used to treat these infections. However, 80-90% of Staphylococci have become resistant by producing beta-lactamases and methicillin resistance is increasing in both coagulase positive and negative Staphylococci. According to a suggestion made by the NCCLS, the use of penicillin and oxacillin is sufficient in predicting susceptibility to all other beta-lactams. In this study, 67 isolates of Staphylococcus including 42 S. aurous, 23 S. epidermidis and 2 S. saprophyticus, were collected from two health centers in Tehran. The antibiotic sensitivity to Penicillin, Ampicillin, Oxacillin, Cephalexin, Cefazolin, Ceftazidime, Amoxicillin-clavulanic acid and Ampicillin-Sulbactam was determined using the disk diffusion method. Beta-lactamase production was also measured by two assays using single colonies, the iodometric technique and nitrocefin disks (cefinase). The results showed that all S. aurous isolates were resistant to penicillin and produced beta-lactamase. Oxacillin resistance was observed in 45.5% of the isolates. Among coagulase-negative staphylococci, 92% were penicillin resistant and 48% were also resistant to oxacillin. Only one oxacillin resistant strain did not produce beta lactamase. 15.2% of MRSA and 58% of MRCNS were sensitive to the other tested beta-lactams.

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APA: Copy

DANESH MONFARD, S., & EFTEKHAR, F.. (2004). EVALUATION OF RESISTANCE TO B-LACTAM ANTIBIOTICS IN STAPHYLOCOCCI USING PENICILLIN AND OXACILLIN DISKS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 8(23), 1-5. SID. https://sid.ir/paper/53015/en

Vancouver: Copy

DANESH MONFARD S., EFTEKHAR F.. EVALUATION OF RESISTANCE TO B-LACTAM ANTIBIOTICS IN STAPHYLOCOCCI USING PENICILLIN AND OXACILLIN DISKS. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2004;8(23):1-5. Available from: https://sid.ir/paper/53015/en

IEEE: Copy

S. DANESH MONFARD, and F. EFTEKHAR, “EVALUATION OF RESISTANCE TO B-LACTAM ANTIBIOTICS IN STAPHYLOCOCCI USING PENICILLIN AND OXACILLIN DISKS,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 8, no. 23, pp. 1–5, 2004, [Online]. Available: https://sid.ir/paper/53015/en

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