IMPROVEMENT IN THE I-PEP METHOD AND ITS EFFECT ON THE OUTCOME OF LOW COPY NUMBER DNA PROFILING

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MOZAFARI ZAHRA; RAHIMI NEJAD FAEZEH; KIYAN FAR SUDEH; B. SARHADDI AMENEH; SHIRKAVAND ATEFEH; MAHDIEH NEJAT; MASHAYEKHI MOHAMMAD R.; GHASEMI AKRAM; TAVALLAEI MAHMOOD; ZEINALI SIROUS

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Journal: Iranian Journal of Forensic Medicine Year:2010 | Volume:16 | Issue:2 (58) Page(s): 107-117

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Title

IMPROVEMENT IN THE I-PEP METHOD AND ITS EFFECT ON THE OUTCOME OF LOW COPY NUMBER DNA PROFILING

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Keywords

FORENSIC MEDICINEQ2

FORENSIC IDENTIFICATIONQ3

DNA PROFILINGQ3

WGAQ3

KI-PEP PCRQ3

Abstract

Background: DNA PROFILING has become one of the most robust and reliable methods at FORENSIC IDENTIFICATION; However, an insufficient DNA quantity (less than 100 Pg or 33 copies) found often in forensic evidence samples, is a major hindrance. Amplification of such low copy number DNA samples is attainable with the most efficient whole genome amplification (WGA) method, named improved primer extension preamplification (I-PEP) PCR.Methods: By initial assessment of existing PEP and I-PEP methods on serially diluted DNA, it was attempted to reach an improved method leading to reliable profiling with the lowest amount of template. This method employs degenerate 15-mer PCR primers followed by specific amplification of DNA with specific primers.Findings: Subsequent to the amplification with the new modified and improved I-PEP, which we term KI-PEP PCR, complete DNA profile was obtained from only 2.5 pg of input DNA. Using this method, a fragment size of 1106 bp was effortlessly amplified with the specific primers.Conclusions: The Utility of KI-PEP PCR not only increases the low quantity of DNA, but also provides the optimum length appropriate to DNA Typing techniques.

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APA: Copy

MOZAFARI, ZAHRA, RAHIMI NEJAD, FAEZEH, KIYAN FAR, SUDEH, B. SARHADDI, AMENEH, SHIRKAVAND, ATEFEH, MAHDIEH, NEJAT, MASHAYEKHI, MOHAMMAD R., GHASEMI, AKRAM, TAVALLAEI, MAHMOOD, & ZEINALI, SIROUS. (2010). IMPROVEMENT IN THE I-PEP METHOD AND ITS EFFECT ON THE OUTCOME OF LOW COPY NUMBER DNA PROFILING. SCIENTIFIC JOURNAL OF FORENSIC MEDICINE, 16(2 (58)), 107-117. SID. https://sid.ir/paper/53268/en

Vancouver: Copy

MOZAFARI ZAHRA, RAHIMI NEJAD FAEZEH, KIYAN FAR SUDEH, B. SARHADDI AMENEH, SHIRKAVAND ATEFEH, MAHDIEH NEJAT, MASHAYEKHI MOHAMMAD R., GHASEMI AKRAM, TAVALLAEI MAHMOOD, ZEINALI SIROUS. IMPROVEMENT IN THE I-PEP METHOD AND ITS EFFECT ON THE OUTCOME OF LOW COPY NUMBER DNA PROFILING. SCIENTIFIC JOURNAL OF FORENSIC MEDICINE[Internet]. 2010;16(2 (58)):107-117. Available from: https://sid.ir/paper/53268/en

IEEE: Copy

ZAHRA MOZAFARI, FAEZEH RAHIMI NEJAD, SUDEH KIYAN FAR, AMENEH B. SARHADDI, ATEFEH SHIRKAVAND, NEJAT MAHDIEH, MOHAMMAD R. MASHAYEKHI, AKRAM GHASEMI, MAHMOOD TAVALLAEI, and SIROUS ZEINALI, “IMPROVEMENT IN THE I-PEP METHOD AND ITS EFFECT ON THE OUTCOME OF LOW COPY NUMBER DNA PROFILING,” SCIENTIFIC JOURNAL OF FORENSIC MEDICINE, vol. 16, no. 2 (58), pp. 107–117, 2010, [Online]. Available: https://sid.ir/paper/53268/en

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