SERODIAGNOSIS OF HERPES SIMPLEX VIRUS-1 INFECTIONS BY A PREPARED ANTIGEN

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SHAHKARAMI M.K.; MIRCHAMCY H.; SHAFIEI A.; KARGAR MOAKHAR R.

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Journal Paper

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2000 | Volume:- | Issue:51 Page(s): 123-127

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Title

SERODIAGNOSIS OF HERPES SIMPLEX VIRUS-1 INFECTIONS BY A PREPARED ANTIGEN

Author(s)

SHAHKARAMI M.K. | MIRCHAMCY H. | SHAFIEI A. | KARGAR MOAKHAR R. | Issue Writer Certificate

Keywords

HERPES SIMPLEX

COMPLEMENT FIXATION TEST

ANTIGEN

Abstract

For diagnosis of HERPES SIMPLEX virus (HSV) primary infections by use of COMPLEMENT FIXATION TEST, the HSV-CF ANTIGEN was prepared. Virus was isolated from herpetic lesions on lips and confirmed by electron microscopy and specific antisera. The results confirmed correct HSV isolation. The isolated virus inoculated to susceptible cell line-Vero-with a high infections dose (MOI=0.1) and incubated at 33°C. When complete CPE appeared, supernatant harvested as source of ANTIGEN.93 serum samples were tested by COMPLEMENT FIXATION TEST (Kolmer method) using both prepared and imported ANTIGENs. The specificity and sensitivity of the test using prepared ANTIGEN in comparison of imported one were 100% and 84.7%, respectively. Because of we prepare the ANTIGEN using only one of two serotypes of the virus, in some cases that antibody directed against other serotype in patient's serum, false-negative results occur. This led to reduction the sensitivity of test by homemade ANTIGEN.

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APA: Copy

SHAHKARAMI, M.K., MIRCHAMCY, H., SHAFIEI, A., & KARGAR MOAKHAR, R.. (2000). SERODIAGNOSIS OF HERPES SIMPLEX VIRUS-1 INFECTIONS BY A PREPARED ANTIGEN. ARCHIVES OF RAZI INSTITUTE, -(51), 123-127. SID. https://sid.ir/paper/535619/en

Vancouver: Copy

SHAHKARAMI M.K., MIRCHAMCY H., SHAFIEI A., KARGAR MOAKHAR R.. SERODIAGNOSIS OF HERPES SIMPLEX VIRUS-1 INFECTIONS BY A PREPARED ANTIGEN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2000;-(51):123-127. Available from: https://sid.ir/paper/535619/en

IEEE: Copy

M.K. SHAHKARAMI, H. MIRCHAMCY, A. SHAFIEI, and R. KARGAR MOAKHAR, “SERODIAGNOSIS OF HERPES SIMPLEX VIRUS-1 INFECTIONS BY A PREPARED ANTIGEN,” ARCHIVES OF RAZI INSTITUTE, vol. -, no. 51, pp. 123–127, 2000, [Online]. Available: https://sid.ir/paper/535619/en

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