GENETIC POLYMORPHISM AT MTNR1A, CAST AND CAPN LOCI IN IRANIAN KARAKUL SHEEP

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EFTEKHARI SHAHROUDI F.; NASIRI M.R.; VALIZADEH R.; HERAVI MOUSAVI A.R.; TAHMOURES POUR M.; GHIASI H.

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Journal Paper

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Journal: Iranian Journal of Biotechnology Year:2006 | Volume:4 | Issue:2 Page(s): 117-122

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Title

GENETIC POLYMORPHISM AT MTNR1A, CAST AND CAPN LOCI IN IRANIAN KARAKUL SHEEP

Author(s)

Keywords

MTNR1A

CALPASTATIN

CALPAIN

POLYMORPHISM

PCR-RFLP

SSCP

KARAKUL SHEEP

Abstract

Genotypes for melatonin receptor type 1A (MTNR1A) and CALPASTATIN (CAST) were determined by enzymatic digestion of PCR products and CALPAIN (CAPN) genotype detected by PCR-SSCP method in Iranian KARAKUL SHEEP. Blood samples were collected from 100 purebred KARAKUL SHEEP. The extraction of genomic DNA was based on guanidinium thiocyanate- silica gel method. PCR amplicons were digested with restriction enzymes Mnll and MspI for MTNR1A and CAST genes, respectively. The MTNR1A locus had two alleles with frequencies of 0.79 for (+) and 0.21 for (-) alleles. Allelic frequencies for CAST locus were 0.85 for M and 0.15 for N. In addition, CALPAIN had two alleles A and B with respective frequencies of 0.79 and 0.21. The observed heterozygosity values for MTNR1A, CALPASTATIN and CALPAIN locus were 0.42, 0.29 and 0.35, respectively. The X2 test confirmed the existence of Hardy-Weinberg equilibrium for the three loci in the population. The data showed a large variation in studied genes. The genetic POLYMORPHISM could be regarded as useful tool for selection programs based on marker- assisted selection between different genotypes of those loci.

Cites

POLYMORPHISM OF SOME GENES ASSOCIATED WITH MEAT‐RELATED TRAITS IN EGYPTIAN SHEEP BREEDS

References

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APA: Copy

EFTEKHARI SHAHROUDI, F., NASIRI, M.R., VALIZADEH, R., HERAVI MOUSAVI, A.R., TAHMOURES POUR, M., & GHIASI, H.. (2006). GENETIC POLYMORPHISM AT MTNR1A, CAST AND CAPN LOCI IN IRANIAN KARAKUL SHEEP. IRANIAN JOURNAL OF BIOTECHNOLOGY, 4(2), 117-122. SID. https://sid.ir/paper/536896/en

Vancouver: Copy

EFTEKHARI SHAHROUDI F., NASIRI M.R., VALIZADEH R., HERAVI MOUSAVI A.R., TAHMOURES POUR M., GHIASI H.. GENETIC POLYMORPHISM AT MTNR1A, CAST AND CAPN LOCI IN IRANIAN KARAKUL SHEEP. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2006;4(2):117-122. Available from: https://sid.ir/paper/536896/en

IEEE: Copy

F. EFTEKHARI SHAHROUDI, M.R. NASIRI, R. VALIZADEH, A.R. HERAVI MOUSAVI, M. TAHMOURES POUR, and H. GHIASI, “GENETIC POLYMORPHISM AT MTNR1A, CAST AND CAPN LOCI IN IRANIAN KARAKUL SHEEP,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 2, pp. 117–122, 2006, [Online]. Available: https://sid.ir/paper/536896/en

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