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Information Journal Paper

Title

DETECTION AND GENOTYPING OF HEPATITIS D VIRUS FROM HBSAG POSITIVE PATIENTS IN IRAN USING RT-PCR

Pages

  174-179

Abstract

HEPATITIS DELTA VIRUS (HDV) is a degenerate RNA virus or virusoid and a satellite of Hepatitis B virus (HBV). Three distinct genotypes are described for HDV, genotype I is distributed worldwide but other genotypes appear to be more restricted geographically. In the present study, an RT-nested PCR method was set up to detect delta infection from serum samples. Moreover, the target amplified sequences corresponding to the Hepatitis delta antigen (HDAg) C-termini were used for genotyping. The results showed that 63.6% (23 of 36) of (HDAb) positive serum samples (as determined by ELISA) were also positive for HDV-RNA. SEQUENCING and phylogenic analysis of three IRANian HDV isolates revealed the most homology (93%) with an Italian isolate indicating a close relationship and probably a common origin for hese isolates.

Cites

References

Cite

APA: Copy

SHAHIN SAZ, L., SABAHI, F., KARIMI, MOHSEN, BEHZADIAN, F., ALAVIAN, S.M., & ZAND, V.. (2006). DETECTION AND GENOTYPING OF HEPATITIS D VIRUS FROM HBSAG POSITIVE PATIENTS IN IRAN USING RT-PCR. IRANIAN JOURNAL OF BIOTECHNOLOGY, 4(3), 174-179. SID. https://sid.ir/paper/551420/en

Vancouver: Copy

SHAHIN SAZ L., SABAHI F., KARIMI MOHSEN, BEHZADIAN F., ALAVIAN S.M., ZAND V.. DETECTION AND GENOTYPING OF HEPATITIS D VIRUS FROM HBSAG POSITIVE PATIENTS IN IRAN USING RT-PCR. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2006;4(3):174-179. Available from: https://sid.ir/paper/551420/en

IEEE: Copy

L. SHAHIN SAZ, F. SABAHI, MOHSEN KARIMI, F. BEHZADIAN, S.M. ALAVIAN, and V. ZAND, “DETECTION AND GENOTYPING OF HEPATITIS D VIRUS FROM HBSAG POSITIVE PATIENTS IN IRAN USING RT-PCR,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 3, pp. 174–179, 2006, [Online]. Available: https://sid.ir/paper/551420/en

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