مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

GENETIC VARIATIONS OF AVIAN PASTEURELLA MULTOCIDA AS DEMONSTRATED BY 16S-23S RRNA GENE SEQUENCES COMPARISON

Pages

  240-244

Abstract

 PASTEURELLA MULTOCIDA is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates. Sequence alignment of the 16S-23S rRNA genes (ITS) revealed a considerable heterogenicity among the isolates. The percentage of similarity varied from 83.3 to 100% among the isolates. An interesting finding from this study was the presence of an inserted sequence (seven nucleotides) in the 16S-23S rRNA region in 55% of the isolates. According to phylogenic analysis based on ITS SEQUENCE alignment, the P. multocida isolates classified into 2 distinct clusters. The virulence of isolates in cluster II were higher than those in cluster I. RIBOTYPING of P. multocida by using 16S-23S rRNA gene PCR sequencing could be used as a marker in epidemiologic studies.

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    • Cite

    APA: Copy

    JABARI, A.R., ESMAEILZADEH, M., MOAZENI JOULA, GH.R., & MOUSAVI SHOUSHTARI, M.. (2008). GENETIC VARIATIONS OF AVIAN PASTEURELLA MULTOCIDA AS DEMONSTRATED BY 16S-23S RRNA GENE SEQUENCES COMPARISON. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), 9(3 (24)), 240-244. SID. https://sid.ir/paper/556382/en

    Vancouver: Copy

    JABARI A.R., ESMAEILZADEH M., MOAZENI JOULA GH.R., MOUSAVI SHOUSHTARI M.. GENETIC VARIATIONS OF AVIAN PASTEURELLA MULTOCIDA AS DEMONSTRATED BY 16S-23S RRNA GENE SEQUENCES COMPARISON. IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR)[Internet]. 2008;9(3 (24)):240-244. Available from: https://sid.ir/paper/556382/en

    IEEE: Copy

    A.R. JABARI, M. ESMAEILZADEH, GH.R. MOAZENI JOULA, and M. MOUSAVI SHOUSHTARI, “GENETIC VARIATIONS OF AVIAN PASTEURELLA MULTOCIDA AS DEMONSTRATED BY 16S-23S RRNA GENE SEQUENCES COMPARISON,” IRANIAN JOURNAL OF VETERINARY RESEARCH (IJVR), vol. 9, no. 3 (24), pp. 240–244, 2008, [Online]. Available: https://sid.ir/paper/556382/en

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