BREAKS IN THE NUCLEOUS OF SACCHAROMYCE CEREVISIAE

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MOTEVALIBASHI MAJID; HOJATI Z.

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Journal: RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE" Year:2005 | Volume:22 | Issue:2 Page(s): 13-24

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Information Journal Paper

Title

BREAKS IN THE NUCLEOUS OF SACCHAROMYCE CEREVISIAE

Author(s)

MOTEVALIBASHI MAJID | HOJATI Z. | Issue Writer Certificate

Keywords

SACCHAROMYCES CEREVISIAEQ4

PULSE FIELD GEL ELECTROPHORESIS (CHEF)Q4

NUCLEOLUSQ4

DOUBLE-STRAND BREAKS (DSB)Q4

RDNA GENEQ4

Abstract

The eukaryotic cells have one or more NUCLEOLUS in the nucleus. SACCHAROMYCES CEREVISIAE has a crescent NUCLEOLUS close to the nuclear envelope into which part of chromosome XII including repeated units of the RDNA GENEs is transported into crescent NUCLEOLUS of yeast. Studies on the recombination at this region show some crossing over between sister chromatid of chromosome XII. However, non-sister chromatid exchange of homologous chromosome XII is not observed at the rDNA region, suggesting in suppression of double-strand breaks (DSBs) at that region. It has been suggested that silencing proteins including Sir2 have important role in silencing state of chromatin for recombination. We studied the occurrence of DSBs at the rDNA region using pulse field gel electrophoresis via CHEF technique and restriction enzymes which cannot cut within the rDNA unit. DSB's fragments were detected using an specific rDNA probe in Southern blot technique. Despite previous suggestions, results show some breaks in the NUCLEOLUS, which cannot be detected using recombination techniques. This suggests that ultra structure of chromatin in the NUCLEOLUS is completely different compared to that of the nucleus.

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APA: Copy

MOTEVALIBASHI, MAJID, & HOJATI, Z.. (2005). BREAKS IN THE NUCLEOUS OF SACCHAROMYCE CEREVISIAE. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", 22(2), 13-24. SID. https://sid.ir/paper/55907/en

Vancouver: Copy

MOTEVALIBASHI MAJID, HOJATI Z.. BREAKS IN THE NUCLEOUS OF SACCHAROMYCE CEREVISIAE. RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE"[Internet]. 2005;22(2):13-24. Available from: https://sid.ir/paper/55907/en

IEEE: Copy

MAJID MOTEVALIBASHI, and Z. HOJATI, “BREAKS IN THE NUCLEOUS OF SACCHAROMYCE CEREVISIAE,” RESEARCH JOURNAL OF UNIVERSITY OF ISFAHAN "SCIENCE", vol. 22, no. 2, pp. 13–24, 2005, [Online]. Available: https://sid.ir/paper/55907/en

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