AN EFFICIENT METHOD FOR DNA EXTRACTION FROM PARAFFIN WAX EMBEDDED TISSUES FOR PCR AMPLIFICATION OF HUMAN AND VIRAL DNA

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TABANIFAR B.; SALEHI RASOUL; ASGARANI E.; FAGHIHI MEHRI; HEYDARPOUR M.; ALAMEH T.A.S.

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Journal Paper

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Journal: Iranian Journal of Pathology Year:2008 | Volume:3 | Issue:4 Page(s): 173-178

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Title

AN EFFICIENT METHOD FOR DNA EXTRACTION FROM PARAFFIN WAX EMBEDDED TISSUES FOR PCR AMPLIFICATION OF HUMAN AND VIRAL DNA

Author(s)

Keywords

DNA

PARAFFIN EMBEDDING

POLYMERASE CHAIN REACTION

Abstract

Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues.Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation.Results: This method produced DNA suitable for amplification using primers specific for human SMN and b globin genes in 98.63% and 82.2% of samples respectively. We also detected human papillomavirus DNA in 58.33% of appropriate samples.Conclusion: This procedure provides simple and efficient method for recovery of amplifiable genomic and viral DNA from paraffin wax embedded tissues.

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APA: Copy

TABANIFAR, B., SALEHI, RASOUL, ASGARANI, E., FAGHIHI, MEHRI, HEYDARPOUR, M., & ALAMEH, T.A.S.. (2008). AN EFFICIENT METHOD FOR DNA EXTRACTION FROM PARAFFIN WAX EMBEDDED TISSUES FOR PCR AMPLIFICATION OF HUMAN AND VIRAL DNA. IRANIAN JOURNAL OF PATHOLOGY (IJP), 3(4), 173-178. SID. https://sid.ir/paper/560387/en

Vancouver: Copy

TABANIFAR B., SALEHI RASOUL, ASGARANI E., FAGHIHI MEHRI, HEYDARPOUR M., ALAMEH T.A.S.. AN EFFICIENT METHOD FOR DNA EXTRACTION FROM PARAFFIN WAX EMBEDDED TISSUES FOR PCR AMPLIFICATION OF HUMAN AND VIRAL DNA. IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2008;3(4):173-178. Available from: https://sid.ir/paper/560387/en

IEEE: Copy

B. TABANIFAR, RASOUL SALEHI, E. ASGARANI, MEHRI FAGHIHI, M. HEYDARPOUR, and T.A.S. ALAMEH, “AN EFFICIENT METHOD FOR DNA EXTRACTION FROM PARAFFIN WAX EMBEDDED TISSUES FOR PCR AMPLIFICATION OF HUMAN AND VIRAL DNA,” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 3, no. 4, pp. 173–178, 2008, [Online]. Available: https://sid.ir/paper/560387/en

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