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Information Journal Paper

Title

POLYMORPHISM OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 IN HOLSTEIN BULLS OF IRAN USING PCR-RFLP

Pages

  197-200

Abstract

 The HOLSTEIN BULLS (n=50) were genotyped for BOVINE LYMPHOCYTE ANTIGEN (BoLA-DRB3.2) alleles by polymerase chain reaction and restriction fragment length polymorphism (PCRRFLP). Genomic DNA was extracted from bull semen using phenol-chloroform method. A two-step PCR was conducted in order to amplify a 284 base-pair fragment of the target gene. Amplicons were digested by RsaI, HaeIII and BstyI restriction endonuclease enzymes. Digested fragments were electrophoresed on 8% polyacrylamide gel and visualized after silver staining. Seventeen BoLA-DRB3.2 alleles were identified with frequencies ranging from 1 to 21%. Sixteen alleles were similar to those reported previously and one was a new allele which has not been reported before. The frequencies of alleles BoLA-DRB3.2 *3, *8, *10, *11, *12, *13, *15, *16, *21, *22, *23, *24, *28, *51, *iaa, *ibb, *qbb were 2, 9, 2, 14, 1, 2, 4, 10, 1, 14, 5, 21, 6, 6, 1, 1, and 1%, respectively. The seven most frequent alleles (BoLA-DRB3.2 *8, *11, *16, *22, *24, *28, *51) accounted for 80% of alleles in the investigated population. This data indicate that the BoLADRB3.2 locus is highly polymorphic in HOLSTEIN BULLS of Iran.

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    Cite

    APA: Copy

    PARNIAN, M., GHOREYSHI, S.A., SALEHI, A.R., PASHMI, M., & MOLA SALEHI, M.R.. (2006). POLYMORPHISM OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 IN HOLSTEIN BULLS OF IRAN USING PCR-RFLP. IRANIAN JOURNAL OF BIOTECHNOLOGY, 4(3), 197-200. SID. https://sid.ir/paper/562831/en

    Vancouver: Copy

    PARNIAN M., GHOREYSHI S.A., SALEHI A.R., PASHMI M., MOLA SALEHI M.R.. POLYMORPHISM OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 IN HOLSTEIN BULLS OF IRAN USING PCR-RFLP. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2006;4(3):197-200. Available from: https://sid.ir/paper/562831/en

    IEEE: Copy

    M. PARNIAN, S.A. GHOREYSHI, A.R. SALEHI, M. PASHMI, and M.R. MOLA SALEHI, “POLYMORPHISM OF BOVINE LYMPHOCYTE ANTIGEN DRB3.2 IN HOLSTEIN BULLS OF IRAN USING PCR-RFLP,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 4, no. 3, pp. 197–200, 2006, [Online]. Available: https://sid.ir/paper/562831/en

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