IMMUNOFLUORESCENT PROTEIN DETECTION IN WESTERN BLOTTING

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NIKBAKHT BROUJENI GH.R.; EMAM M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Veterinary Medicine Year:2009 | Volume:3 | Issue:2 Page(s): 107-111

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Information Journal Paper

Title

IMMUNOFLUORESCENT PROTEIN DETECTION IN WESTERN BLOTTING

Author(s)

NIKBAKHT BROUJENI GH.R. | EMAM M. | Issue Writer Certificate

Keywords

IMMUNOFLUORESCENCE

IMMUNOBLOTTING

WESTERN BLOTTING

FITC

PVDF

Abstract

This report describes the detailed procedures for Western blot analysis using fluorescent antibodies. After electrophoresis and subsequent electroblotting, the fluorescentlabeled antibodies were visible upon ultraviolet illumination of the polyvinylidene fluoride (PVDF) membranes and could then be photographed to give an accurate record of the blots. Fluorescent labeling allows for photographic records of the blot without the need for subsequent staining steps. In an effort to evaluate this method in the study of immunogenic antigens, this study describes a study on BLV (Bovine Leukemia Virus) tumor antigens. The fluorescent labeling of antibodies for detection by IMMUNOBLOTTING was found to be a very sensitive and reliable alternative to the conventional methods of Western blots.

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APA: Copy

NIKBAKHT BROUJENI, GH.R., & EMAM, M.. (2009). IMMUNOFLUORESCENT PROTEIN DETECTION IN WESTERN BLOTTING. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 3(2), 107-111. SID. https://sid.ir/paper/568929/en

Vancouver: Copy

NIKBAKHT BROUJENI GH.R., EMAM M.. IMMUNOFLUORESCENT PROTEIN DETECTION IN WESTERN BLOTTING. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2009;3(2):107-111. Available from: https://sid.ir/paper/568929/en

IEEE: Copy

GH.R. NIKBAKHT BROUJENI, and M. EMAM, “IMMUNOFLUORESCENT PROTEIN DETECTION IN WESTERN BLOTTING,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 3, no. 2, pp. 107–111, 2009, [Online]. Available: https://sid.ir/paper/568929/en

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