IMPROVED PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LEAF TISSUES OF PHYLLANTHUS EMBLICA GAERTN ?(SHORT COMMUNICATION)

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NAGARAJAN SANGEETHA; STEEPHEN MERCY; MURUGAN KAVITHA; RAHUL RAVEENDRAN NAIR; SETHURAMAN THILAGA; ALAGAR PARAMESWARI; GANESH DOSS

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Journal: Iranian Journal of Biotechnology Year:2011 | Volume:9 | Issue:4 Page(s): 307-313

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Title

IMPROVED PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LEAF TISSUES OF PHYLLANTHUS EMBLICA GAERTN ?(SHORT COMMUNICATION)

Author(s)

Keywords

PHYLLANTHUS EMBLICA

CHLOROPLAST DNA

RESTRICTION ENZYMES

TRNL (UAA) INTRON SEQUENCES

GENE SPECIFIC PRIMERS

Abstract

Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissues of PHYLLANTHUS EMBLICA for obtaining high quality genomic DNA. Fresh leaves of three different maturity were analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pH was the primary cause for poor quality of DNA.However, high quality DNA isolation was achieved by stabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocol yielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes for molecular analysis.

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APA: Copy

NAGARAJAN, SANGEETHA, STEEPHEN, MERCY, MURUGAN, KAVITHA, RAHUL RAVEENDRAN, NAIR, SETHURAMAN, THILAGA, ALAGAR, PARAMESWARI, & GANESH, DOSS. (2011). IMPROVED PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LEAF TISSUES OF PHYLLANTHUS EMBLICA GAERTN ?(SHORT COMMUNICATION). IRANIAN JOURNAL OF BIOTECHNOLOGY, 9(4), 307-313. SID. https://sid.ir/paper/573894/en

Vancouver: Copy

NAGARAJAN SANGEETHA, STEEPHEN MERCY, MURUGAN KAVITHA, RAHUL RAVEENDRAN NAIR, SETHURAMAN THILAGA, ALAGAR PARAMESWARI, GANESH DOSS. IMPROVED PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LEAF TISSUES OF PHYLLANTHUS EMBLICA GAERTN ?(SHORT COMMUNICATION). IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2011;9(4):307-313. Available from: https://sid.ir/paper/573894/en

IEEE: Copy

SANGEETHA NAGARAJAN, MERCY STEEPHEN, KAVITHA MURUGAN, NAIR RAHUL RAVEENDRAN, THILAGA SETHURAMAN, PARAMESWARI ALAGAR, and DOSS GANESH, “IMPROVED PROTOCOL FOR ISOLATION OF GENOMIC DNA FROM LEAF TISSUES OF PHYLLANTHUS EMBLICA GAERTN ?(SHORT COMMUNICATION),” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 9, no. 4, pp. 307–313, 2011, [Online]. Available: https://sid.ir/paper/573894/en

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