EFFECT OF PHYTOHORMONE AND EXPLANT ON CALLUGENESIS AND REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA L.)

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GARSHASBI H.; OMIDI MANSOUR; TORABI SEPIDEH; DAVOUDI D.

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Journal: Iranian Journal of Crop Sciences Year:2009 | Volume:11 | Issue:2 (42) Page(s): 101-108

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Title

EFFECT OF PHYTOHORMONE AND EXPLANT ON CALLUGENESIS AND REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA L.)

Author(s)

GARSHASBI H. | OMIDI MANSOUR | TORABI SEPIDEH | DAVOUDI D. | Issue Writer Certificate

Keywords

CALLUGENESISQ3

EXPLANTQ2

PHYTOHORMONEQ2

REGENERATION AND SAINFOINQ3

Abstract

To study the effects of PHYTOHORMONE and EXPLANTs on the CALLUGENESIS and regeneration of Sainfoin (Onobrychis sativa L. var. Chadegan) an experiment was conducted in the biotechnology laboratory of Sciences and Research Unit, lslamic Azad University, Tehran, Iran in summer and spring of 2006. In this experiment, two PHYTOHORMONEs (2,4-D and BAP) and 4 EXPLANTs (cotyledon leaf, apical meristem, stem, and root) were used. The highest number of callus was obtained on the MS medium containing 2,4-D (4 mg.l-1) + BAP (1.5mg.l-1) + apical meristem EXPLANT and 2,4-D (2mg.l-1) + BAP (1mg.l-1) + apical meristem EXPLANT with means of 1.77 and 1.76 callus, respectively. The highest regeneration was obtained in MS medium containing BAP (4 mg mg.l-1) with mean of 1.6 regenerated plants. A new method for in vitro micropropagation of Sainfoin (Onobrychis sativa Var Chadegan) was developed. Seedlings with root were transferred to greenhouse and continued growing.

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APA: Copy

GARSHASBI, H., OMIDI, MANSOUR, TORABI, SEPIDEH, & DAVOUDI, D.. (2009). EFFECT OF PHYTOHORMONE AND EXPLANT ON CALLUGENESIS AND REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA L.). IRANIAN JOURNAL OF CROP SCIENCES, 11(2 (42)), 101-108. SID. https://sid.ir/paper/57407/en

Vancouver: Copy

GARSHASBI H., OMIDI MANSOUR, TORABI SEPIDEH, DAVOUDI D.. EFFECT OF PHYTOHORMONE AND EXPLANT ON CALLUGENESIS AND REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA L.). IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2009;11(2 (42)):101-108. Available from: https://sid.ir/paper/57407/en

IEEE: Copy

H. GARSHASBI, MANSOUR OMIDI, SEPIDEH TORABI, and D. DAVOUDI, “EFFECT OF PHYTOHORMONE AND EXPLANT ON CALLUGENESIS AND REGENERATION OF SAINFOIN (ONOBRYCHIS SATIVA L.),” IRANIAN JOURNAL OF CROP SCIENCES, vol. 11, no. 2 (42), pp. 101–108, 2009, [Online]. Available: https://sid.ir/paper/57407/en

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