DEVELOPMENT OF PCR BASED MOLECULAR MARKER FOR CANDIDATE FUSARIUM HEAD BLIGHT RESISTANCE GENE (PDR5) IN WHEAT

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EHYA F.; GHAFARI M.R.; MARDI M.; ZAHERI SABOURAN; GHARAHYAZI BEHZAD

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ISSN: 2588-4824

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Journal: Iranian Journal of Crop Sciences Year:2005 | Volume:6 | Issue:4 Page(s): 395-401

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Title

DEVELOPMENT OF PCR BASED MOLECULAR MARKER FOR CANDIDATE FUSARIUM HEAD BLIGHT RESISTANCE GENE (PDR5) IN WHEAT

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Keywords

WHEATQ3

FUSARIUM HEAD BLIGHTQ3

PCRQ3

PDR5 GENEQ4

PHENOTYPINGQ2

GENOTYPINGQ2

Abstract

The PDR5 (pleiotropic drug resistance 5) gene, encoding ABC transporter protein, detoxifies cells using ATP hydrolysis. To develop PCR based marker for FUSARIUM HEAD BLIGHT resistance candidate gene (PDR5) in WHEAT, similar sequences of PDR5 were aligned with DNASTAR software. Primers were designed based on conserved and non conserved sequences. Molecular analysis using moderately resistant (Frontana) and susceptible (Falat) parental lines showed a specific PDR5 allele in Frontana. Amplified allele was isolated, cloned and sequenced. The sequencing was verified on data Bank. Single marker analysis based on PHENOTYPING and GENOTYPING data of F3 generation developed from a cross between Frontana and Falat indicated the specific PDR5 allele had negative effect on FHB infection in WHEAT.

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APA: Copy

EHYA, F., GHAFARI, M.R., MARDI, M., ZAHERI, SABOURAN, & GHARAHYAZI, BEHZAD. (2005). DEVELOPMENT OF PCR BASED MOLECULAR MARKER FOR CANDIDATE FUSARIUM HEAD BLIGHT RESISTANCE GENE (PDR5) IN WHEAT. IRANIAN JOURNAL OF CROP SCIENCES, 6(4), 395-401. SID. https://sid.ir/paper/57422/en

Vancouver: Copy

EHYA F., GHAFARI M.R., MARDI M., ZAHERI SABOURAN, GHARAHYAZI BEHZAD. DEVELOPMENT OF PCR BASED MOLECULAR MARKER FOR CANDIDATE FUSARIUM HEAD BLIGHT RESISTANCE GENE (PDR5) IN WHEAT. IRANIAN JOURNAL OF CROP SCIENCES[Internet]. 2005;6(4):395-401. Available from: https://sid.ir/paper/57422/en

IEEE: Copy

F. EHYA, M.R. GHAFARI, M. MARDI, SABOURAN ZAHERI, and BEHZAD GHARAHYAZI, “DEVELOPMENT OF PCR BASED MOLECULAR MARKER FOR CANDIDATE FUSARIUM HEAD BLIGHT RESISTANCE GENE (PDR5) IN WHEAT,” IRANIAN JOURNAL OF CROP SCIENCES, vol. 6, no. 4, pp. 395–401, 2005, [Online]. Available: https://sid.ir/paper/57422/en

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