IDENTIFICATION OF MYCOBACTERIA SPECIES IN CUTANEOUS LESIONS OF SARCOIDOSIS BY PCR-RESTRICTION RAGMENT
LENGTH POLYMORPHISM (PCR-RFLP) METHOD

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VALIKHANI M.; MIRSALEHIAN A.; MORTAZAVI H.; MANSOURI DAVOUD; POURAKBARI B.; MAHTAPOUR F.

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Journal: Iranian Journal of Dermatology Year:2004 | Volume:3 | Issue:3 (27) Page(s): 166-170

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Title

IDENTIFICATION OF MYCOBACTERIA SPECIES IN CUTANEOUS LESIONS OF SARCOIDOSIS BY PCR-RESTRICTION RAGMENT LENGTH POLYMORPHISM (PCR-RFLP) METHOD

Author(s)

Keywords

SARCOIDOSISQ4

MYCOBACTERIUMQ4

SKIN BIOPSYQ4

Abstract

Background: SARCOIDOSIS is a granulomatous multisystem disease of unknown etiology. It has recently been tried to detect mycobacterial genome in biopsy specimens of patients with SARCOIDOSIS by Polymerase Chain Reaction method. Objective: To detect and identify mycobacteria species in cutaneous lesions of the patients with SARCOIDOSIS by PCR-RFLP. Materials and Methods: Twenty patients with clinical diagnosis of SARCOIDOSIS were enrolled in this study. Clinical manifestations, appearance of naked granuloma under light microscope and exclusion of other diagnoses confirmed the diagnosis of SARCOIDOSIS in the patients. By PCR-RFLP, genome of mycobacteria species was searched in paraffin embedded specimen of skin biopsies of the patients. Four PCR positive SKIN BIOPSY specimens of patients with cutaneous tuberculosis were used as positive control. Ten SKIN BIOPSY specimens with diagnoses other than tuberculosis were used as negative control. Results: Mycobacteria genome was not detected in any specimens of the patients. Conclusion: Our findings do not support the role of mycobacteria species in the pathogenesis of SARCOIDOSIS.

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APA: Copy

VALIKHANI, M., MIRSALEHIAN, A., MORTAZAVI, H., MANSOURI, DAVOUD, POURAKBARI, B., & MAHTAPOUR, F.. (2004). IDENTIFICATION OF MYCOBACTERIA SPECIES IN CUTANEOUS LESIONS OF SARCOIDOSIS BY PCR-RESTRICTION RAGMENT LENGTH POLYMORPHISM (PCR-RFLP) METHOD. IRANIAN JOURNAL OF DERMATOLOGY, 3(3 (27)), 166-170. SID. https://sid.ir/paper/57662/en

Vancouver: Copy

VALIKHANI M., MIRSALEHIAN A., MORTAZAVI H., MANSOURI DAVOUD, POURAKBARI B., MAHTAPOUR F.. IDENTIFICATION OF MYCOBACTERIA SPECIES IN CUTANEOUS LESIONS OF SARCOIDOSIS BY PCR-RESTRICTION RAGMENT LENGTH POLYMORPHISM (PCR-RFLP) METHOD. IRANIAN JOURNAL OF DERMATOLOGY[Internet]. 2004;3(3 (27)):166-170. Available from: https://sid.ir/paper/57662/en

IEEE: Copy

M. VALIKHANI, A. MIRSALEHIAN, H. MORTAZAVI, DAVOUD MANSOURI, B. POURAKBARI, and F. MAHTAPOUR, “IDENTIFICATION OF MYCOBACTERIA SPECIES IN CUTANEOUS LESIONS OF SARCOIDOSIS BY PCR-RESTRICTION RAGMENT LENGTH POLYMORPHISM (PCR-RFLP) METHOD,” IRANIAN JOURNAL OF DERMATOLOGY, vol. 3, no. 3 (27), pp. 166–170, 2004, [Online]. Available: https://sid.ir/paper/57662/en

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