EVALUATION OF PROTECTIVITY AND IMMUNOGENICITY OF ALUM PRECIPITATED AUTOCLAVED LEISHMANIA MAJOR
VACCINE MIXED WITH MYCOBACTERIUM VACCAE IN THE MURINE MODEL OF LEISHMANIASIS

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NATEGHI ROSTAMI M.; KESHAVARZ H.; MIRAMIN MOHAMMADI A.; KHAMESIPOUR A.

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Journal: Iranian Journal of Dermatology Year:2005 | Volume:8 | Issue: 5 (33) Page(s): 363-372

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Title

EVALUATION OF PROTECTIVITY AND IMMUNOGENICITY OF ALUM PRECIPITATED AUTOCLAVED LEISHMANIA MAJOR VACCINE MIXED WITH MYCOBACTERIUM VACCAE IN THE MURINE MODEL OF LEISHMANIASIS

Author(s)

NATEGHI ROSTAMI M. | KESHAVARZ H. | MIRAMIN MOHAMMADI A. | KHAMESIPOUR A. | Issue Writer Certificate

Keywords

L.MAJORQ3

M.VACCAEQ4

ALUMQ4

LEISHMANIAQ3

VACCINATIONQ4

Abstract

Background and Objective: LEISHMANIAses represent a group of diseases caused by protozoan parasite of the genus LEISHMANIA. Control strategies are not always effective; it seems that the sole control measure is to search for an effective vaccine. The objective of this study was evaluation of the rate of protection and immune response induction in Balb/c mice immunized with ALUM precipitated autoclaved LEISHMANIA major (Alum-ALM) vaccine mixed with M. vaccae. Materials and Methods: Eleven groups of female 8-10 week old Balb/c mice were immunized subcutaneously (SC) three times, 21 days apart, with different doses of ALUM-ALM mixed with different doses of either M. vaccae or BCG. The immunized animals and control group were challenged with 1 ×106 L. major. Development and progression of LEISHMANIA infection were assessed by footpad swelling measurement at the site of challenge and parasite burden in lymph nodes. The immune responses of vaccinated animals were evaluated in vivo by leishmanin skin test (LST) and in vitro by measurement of cytokine (IFN-y and IL-4) levels in mononuclear cell culture supematants and titration of serum anti-leishmania antibodies (IgG and its sub-classes). Results: Footpad thickness measurement after challenge with live L. major showed no significant difference between immunized groups and control group. However, there were some prominent exceptional cases in the parasite burden titration in groups 1,4,6, and 8. Immunization with low dose of ALUM-ALM mixed with M. vaccae or BCG induced IFN-yproduction, and diminished IL-4 level (in vitro), and caused a stronger LST response in a group that received BCG as and adjuvant. Mice that were immunized with high doses of ALUM-ALM mixed with high doses of M. vaccae showed an increase in footpad thickness at the site of challenge and higher levels of IL-4 and IgG1. Conclusion: It seems that immunization of mice with a low dose of ALUM-ALM mixed with M. vaccae as an adjuvant might induce a Th1 type response. M.VACCAE mixed with low dose of ALUM- ALM has an inhibitory influence on the parasite burden in the infected tissues of mice. Also, BCG mixed with different doses of ALUM-ALM induces a Thl immune response in Balb/c mice. Apparently, there is n? significant difference in adjuvancity of BCG and M. vaccae. High dose of ALUM-ALM mixed with high dose of M. vaccae induces a Th 2 response.

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APA: Copy

NATEGHI ROSTAMI, M., KESHAVARZ, H., MIRAMIN MOHAMMADI, A., & KHAMESIPOUR, A.. (2005). EVALUATION OF PROTECTIVITY AND IMMUNOGENICITY OF ALUM PRECIPITATED AUTOCLAVED LEISHMANIA MAJOR VACCINE MIXED WITH MYCOBACTERIUM VACCAE IN THE MURINE MODEL OF LEISHMANIASIS. IRANIAN JOURNAL OF DERMATOLOGY, 8(5 (33)), 363-372. SID. https://sid.ir/paper/57933/en

Vancouver: Copy

NATEGHI ROSTAMI M., KESHAVARZ H., MIRAMIN MOHAMMADI A., KHAMESIPOUR A.. EVALUATION OF PROTECTIVITY AND IMMUNOGENICITY OF ALUM PRECIPITATED AUTOCLAVED LEISHMANIA MAJOR VACCINE MIXED WITH MYCOBACTERIUM VACCAE IN THE MURINE MODEL OF LEISHMANIASIS. IRANIAN JOURNAL OF DERMATOLOGY[Internet]. 2005;8(5 (33)):363-372. Available from: https://sid.ir/paper/57933/en

IEEE: Copy

M. NATEGHI ROSTAMI, H. KESHAVARZ, A. MIRAMIN MOHAMMADI, and A. KHAMESIPOUR, “EVALUATION OF PROTECTIVITY AND IMMUNOGENICITY OF ALUM PRECIPITATED AUTOCLAVED LEISHMANIA MAJOR VACCINE MIXED WITH MYCOBACTERIUM VACCAE IN THE MURINE MODEL OF LEISHMANIASIS,” IRANIAN JOURNAL OF DERMATOLOGY, vol. 8, no. 5 (33), pp. 363–372, 2005, [Online]. Available: https://sid.ir/paper/57933/en

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