IMPROVED WHOLE GENOME AMPLIFICATION PROTOCOL AT SINGLE CELL LEVEL

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GALEHDARI HAMID; FOROUGHMAND A.M.; ANDASHTI B.

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Paper Information

Journal: SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE Year:2006 | Volume:NEW SERIES | Issue:15 (SECTION B) Page(s): 35-44

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Information Journal Paper

Title

IMPROVED WHOLE GENOME AMPLIFICATION PROTOCOL AT SINGLE CELL LEVEL

Author(s)

GALEHDARI HAMID | FOROUGHMAND A.M. | ANDASHTI B. | Issue Writer Certificate

Keywords

WGAQ3

DOP-PCRQ3

PEP-PCRQ4

Abstract

Genetic analysis of limited amounts of genomic DNA plays an important role in many molecular biology investigations. A prerequisite for molecular analysis of single or few cells is a reliable and reproducible method for DNA preparation for subsequent polymerase chain reaction (PCR) analyses. We investigated the use of degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) to generate a template for LOH (loss of heterozygosity)- methodology and mutation detection. DOP-PCR employs a degenerate primer to produce non-specific uniform amplification of DNA. This approach has been successfully applied to microsatellite analyzing. We compared amplified whole genome performed by DOP-PCR to the genomic DNA as a template. Results were analyzed with respect to feasibility, amplification efficiency and reproducibility. DOP-PCR yielded overall satisfactory results. Accuracy and quality of genotypes generated from the DOP-PCR template also depends on several conditions such as the choice of DNA polymerase and cell treatment. The conclusion is that we have successfully used DOP-PCR to amplify our genomic DNA from single cell for subsequent genotyping and mutation analysis as a standard process.

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APA: Copy

GALEHDARI, HAMID, FOROUGHMAND, A.M., & ANDASHTI, B.. (2006). IMPROVED WHOLE GENOME AMPLIFICATION PROTOCOL AT SINGLE CELL LEVEL. SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE, NEW SERIES(15 (SECTION B)), 35-44. SID. https://sid.ir/paper/57997/en

Vancouver: Copy

GALEHDARI HAMID, FOROUGHMAND A.M., ANDASHTI B.. IMPROVED WHOLE GENOME AMPLIFICATION PROTOCOL AT SINGLE CELL LEVEL. SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE[Internet]. 2006;NEW SERIES(15 (SECTION B)):35-44. Available from: https://sid.ir/paper/57997/en

IEEE: Copy

HAMID GALEHDARI, A.M. FOROUGHMAND, and B. ANDASHTI, “IMPROVED WHOLE GENOME AMPLIFICATION PROTOCOL AT SINGLE CELL LEVEL,” SHAHID CHAMRAN UNIVERSITY JOURNAL OF SCIENCE, vol. NEW SERIES, no. 15 (SECTION B), pp. 35–44, 2006, [Online]. Available: https://sid.ir/paper/57997/en

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