EVALUATION OFPDX-1 GENE EXPRESSION IN INSULIN PRODUCING CELLS, DERIVED FROM EMBRYONAL CARCINOMA STEM CELLS

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MANSOURI BIDEKANI A.; ESMAEILI F.; HOUSHMAND F.; HAJISHARIFI Z.

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Journal: Journal of Shahrekord University of Medical Sciences Year:2013 | Volume:15 | Issue:1 Page(s): 91-102

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Information Journal Paper

Title

EVALUATION OFPDX-1 GENE EXPRESSION IN INSULIN PRODUCING CELLS, DERIVED FROM EMBRYONAL CARCINOMA STEM CELLS

Author(s)

MANSOURI BIDEKANI A. | ESMAEILI F. | HOUSHMAND F. | HAJISHARIFI Z. | Issue Writer Certificate

Keywords

β RECEPTORQ2

CONDITIONED MEDIUMQ2

STEM CELLSQ2

INSULIN-PROINSULINQ2

PDX - 1Q2

Abstract

Background and aims: Understanding b-cell function at the molecular level will likely facilitate the development of b-cells manufacture techniques. The aim of the present study was to investigate the pancreatic and duodenal homeobox 1 (pdx-1) gene expression into DTZ stained cellular clusters originating from emberyonal carcinoma cells.Methods: This implemental -fundamental study was accomplished on differentiation of STEM CELLS into insulin producing cells (IPCs). The CONDITIONED MEDIUM of cultured pancreas from one-week newborn mouse was used for differentiation of P19. EBs formed by a 24h suspension culture of P19 EC cells. In order to induce the difrentiation,, different concentrations of CONDITIONED MEDIUM (25%, 50%, 75% and 100%) were added to culture medium. Dithizone staininig was used for detecting the differentiated cells derived from EB sin vitro. Insulinproinsulin production and insulin receptor beta were determined by immunofluorescence. The expression of pdx-1 gene was also analyzed by reverse transcriptase-polymerase chain reaction. Data were analyzed using one way ANOVA and Dunkana test.Results: Differentiated cell clusters appeared after approximately 7 days induction. The peak response of differentiation was at the concentration of 50% CONDITIONED MEDIUM. Expression of pdx-1gene was observed in the differentiated cell clusters. Expression of INSULIN-PROINSULIN and insulin receptor beta markers in the differentiated cells was confirmed by immunofluorescence.Conclusion: The pancreas CONDITIONED MEDIUM could influence P19 cells differentiation into insulin producing cells. So findings of this study could facilitate producing the b-cells.

Cites

SURVIVAL AND PROLIFERATION OF PANCREATIC ISLETS ISOLATED FROM ADULT MOUSE IN LABORATORY CULTURE CONDITIONS

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APA: Copy

MANSOURI BIDEKANI, A., ESMAEILI, F., HOUSHMAND, F., & HAJISHARIFI, Z.. (2013). EVALUATION OFPDX-1 GENE EXPRESSION IN INSULIN PRODUCING CELLS, DERIVED FROM EMBRYONAL CARCINOMA STEM CELLS. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 15(1), 91-102. SID. https://sid.ir/paper/58393/en

Vancouver: Copy

MANSOURI BIDEKANI A., ESMAEILI F., HOUSHMAND F., HAJISHARIFI Z.. EVALUATION OFPDX-1 GENE EXPRESSION IN INSULIN PRODUCING CELLS, DERIVED FROM EMBRYONAL CARCINOMA STEM CELLS. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;15(1):91-102. Available from: https://sid.ir/paper/58393/en

IEEE: Copy

A. MANSOURI BIDEKANI, F. ESMAEILI, F. HOUSHMAND, and Z. HAJISHARIFI, “EVALUATION OFPDX-1 GENE EXPRESSION IN INSULIN PRODUCING CELLS, DERIVED FROM EMBRYONAL CARCINOMA STEM CELLS,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 1, pp. 91–102, 2013, [Online]. Available: https://sid.ir/paper/58393/en

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