MOLECULAR CLONING AND EXPRESSION OF BACILLUS ANTHRACIS LETHAL FACTOR DOMAIN 1 GENE IN ESCHERICHIA COLI

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REZAEE M.; HONARI H.; ZAND A.M.

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Paper Information

Journal: Journal of Shahrekord University of Medical Sciences Year:2012 | Volume:14 | Issue:4 Page(s): 38-46

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Title

MOLECULAR CLONING AND EXPRESSION OF BACILLUS ANTHRACIS LETHAL FACTOR DOMAIN 1 GENE IN ESCHERICHIA COLI

Author(s)

REZAEE M. | HONARI H. | ZAND A.M. | Issue Writer Certificate

Keywords

ANTHRAXQ2

BACILLUS ANTHRACISQ2

LETHAL FACTOR DOMAIN 1Q3

RECOMBINANT PROTEINQ2

Abstract

Background and aims: ANTHRAX is a common disease in human and livestock caused by BACILLUS ANTHRACIS. BACILLUS ANTHRACIShas two strong immunogen proteins: Protective antigen (PA) and lethal factor domain I (LFD1) that has been always considered as a candidate vaccine against BACILLUS ANTHRACIS. The aim of this study was to express the lethal factor domain I in Escherichia coli.Methods: In this laboratory experimental study, the gene of LFD1 was detected and amplified from pXO1 plasmid by PCR. The gene was cloned with Bam H I and Xho I restriction site in cloning vector (pGEM-T easy), after isolation was sub cloned to expression vector pET28a (+).This vector was transformed to E. coli -BL21 (DE3) to express LFD1 gene. The expression of LFD1 gene was induced by IPTG, and LFD1 protein was produced.Results: The cloned LFD1 gene in pET28a (+) vector was confirmed by sequencing, PCR and enzymatic analysis. The expressed RECOMBINANT PROTEIN was confirmed by SDS-PAGE and Western blotting.Conclusion: According to immunogenicity of LFD1 protein, the produced RECOMBINANT PROTEIN can be used separately or in combination by adjuvants and delivers to design a vaccine against ANTHRAX.

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APA: Copy

REZAEE, M., HONARI, H., & ZAND, A.M.. (2012). MOLECULAR CLONING AND EXPRESSION OF BACILLUS ANTHRACIS LETHAL FACTOR DOMAIN 1 GENE IN ESCHERICHIA COLI. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 14(4), 38-46. SID. https://sid.ir/paper/58620/en

Vancouver: Copy

REZAEE M., HONARI H., ZAND A.M.. MOLECULAR CLONING AND EXPRESSION OF BACILLUS ANTHRACIS LETHAL FACTOR DOMAIN 1 GENE IN ESCHERICHIA COLI. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2012;14(4):38-46. Available from: https://sid.ir/paper/58620/en

IEEE: Copy

M. REZAEE, H. HONARI, and A.M. ZAND, “MOLECULAR CLONING AND EXPRESSION OF BACILLUS ANTHRACIS LETHAL FACTOR DOMAIN 1 GENE IN ESCHERICHIA COLI,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 14, no. 4, pp. 38–46, 2012, [Online]. Available: https://sid.ir/paper/58620/en

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