ISOLATION AND IDENTIFICATION OF INHIBITORY BACTERIA AGAINST PATHOGENIC FUNGI FROM ISFAHAN USING MOLECULAR METHOD

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NAGHDIFAR SH.; MADANI M.; AHADI A.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Journal of Shahrekord University of Medical Sciences Year:2016 | Volume:18 | Issue:5 Page(s): 83-93

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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF INHIBITORY BACTERIA AGAINST PATHOGENIC FUNGI FROM ISFAHAN USING MOLECULAR METHOD

Author(s)

NAGHDIFAR SH. | MADANI M. | AHADI A.M. | Issue Writer Certificate

Keywords

BACILLUS ATROPHAEUSQ1

PATHOGENIC FUNGIQ1

ANTIFUNGAL ACTIVITYQ3

COLONY-PCRQ1

Abstract

This study was aimed to identify and isolate inhibitory bacterium against PATHOGENIC FUNGI in Isfahan using molecular method.Methods: In this cross-section descriptive, 150 samples (soil, air, and surface) from different parts of Isfahan were prepared and investigated the effect of inhibitory bacteria grown on nutrient agar medium on the growth of Aspergillus niger, Aspergillus flavus and Mucor hiemali. Qualitative investigation of fungal growth inhibition was done by inoculation methods, and quantitative evaluation was conducted using linear culturing in distance of 0.5, 1, 1.5, 2, 2.5 and 3 cm of fungal suspension (104 spore/μl) from the center of plates (bacterial inoculation, 0.5 Macfarland). Samples were incubated in 30ͦC for 96 hours and identification of the inhibitory bacterium was done according to biochemical tests and molecular method of COLONY-PCR.Results: The effect of inhibitory bacteria against Aspergillus niger, Aspergillus flavus and Mucor hiemaliwas from 0.5 to 3 cm. According to biochemical tests and COLONY-PCR, the bacterium with the most inhibitory effect wasBACILLUS ATROPHAEUS strain HNSQJYH170.Conclusion: BACILLUS ATROPHAEUS strain HNSQJYH170 is endemic in Isfahan and can be used in antibiotic production and biological control.

Cites

The Effect of Cyclic Lipopeptides of Bacillus atrophaeus (HNSQJYH170) on Candida Species

References

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APA: Copy

NAGHDIFAR, SH., MADANI, M., & AHADI, A.M.. (2016). ISOLATION AND IDENTIFICATION OF INHIBITORY BACTERIA AGAINST PATHOGENIC FUNGI FROM ISFAHAN USING MOLECULAR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 18(5), 83-93. SID. https://sid.ir/paper/58647/en

Vancouver: Copy

NAGHDIFAR SH., MADANI M., AHADI A.M.. ISOLATION AND IDENTIFICATION OF INHIBITORY BACTERIA AGAINST PATHOGENIC FUNGI FROM ISFAHAN USING MOLECULAR METHOD. JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;18(5):83-93. Available from: https://sid.ir/paper/58647/en

IEEE: Copy

SH. NAGHDIFAR, M. MADANI, and A.M. AHADI, “ISOLATION AND IDENTIFICATION OF INHIBITORY BACTERIA AGAINST PATHOGENIC FUNGI FROM ISFAHAN USING MOLECULAR METHOD,” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 18, no. 5, pp. 83–93, 2016, [Online]. Available: https://sid.ir/paper/58647/en

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