NATURAL CYTOTOXICITY (NC) OF IL-3 INDUCED MAST CELL LINE IS NOT MEDIATED BY TUMOR NECROSIS FACTOR-ALPHA (TNF-Α)

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SHIRZADEH H.A.

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Journal Paper

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Journal: Journal of Shahrekord University of Medical Sciences Year:2002 | Volume:4 | Issue:1 Page(s): 33-37

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Title

NATURAL CYTOTOXICITY (NC) OF IL-3 INDUCED MAST CELL LINE IS NOT MEDIATED BY TUMOR NECROSIS FACTOR-ALPHA (TNF-Α)

Author(s)

SHIRZADEH H.A. | Issue Writer Certificate

Keywords

INTERLEUKIN-3Q4

TUMOR NECROSIS FACTOR ALPHAQ4

NATURAL CYTOTOXICITYQ4

Abstract

Long-term culture of CBA mouse spleen cells in IL-3 conditioned medium induced a stable mast cell line (MCL). This mast cell line expressed NC-I.I receptor that mediates NATURAL CYTOTOXICITY against tumor, but had no markers of T cell, B cells, macrophages, or NK cells. Since in most literature TUMOR NECROSIS FACTOR ALPHA (TNF-α) has been proposed as a major mediator in NATURAL CYTOTOXICITY, and MCL cells has no receptor for TNF-α, in this", study we aimed to identify the role of TNF- α in killing of WEHI-164 fibrosarcoma by MCL. MCL and spleen cells from mice (CBAxC57BL/6) F1 (Control group) were separately incubated with anti-TNF-a antibody to block the NC. Furthermore to investigate the presence of TNF- α gene in MCL, mRNA of TNF- α gene was studied using RT-PCR experiment. Results showed that NATURAL CYTOTOXICITY of MCL was not blocked by anti-TNF- α compared to that of F1 mice spleen cells (CBAxC57BL/6) Fl. Further RT-PCR results showed no constitutive expression of TNF- α mRNA in MCL and upon interaction with WEHI-164 targets, only transient induction of TNF- a mRNA was observed at 30 minutes. We conclude that the NC activity of MCL is mediated by factor(s) other than TNF - α.

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APA: Copy

SHIRZADEH, H.A.. (2002). NATURAL CYTOTOXICITY (NC) OF IL-3 INDUCED MAST CELL LINE IS NOT MEDIATED BY TUMOR NECROSIS FACTOR-ALPHA (TNF-?). JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, 4(1), 33-37. SID. https://sid.ir/paper/58796/en

Vancouver: Copy

SHIRZADEH H.A.. NATURAL CYTOTOXICITY (NC) OF IL-3 INDUCED MAST CELL LINE IS NOT MEDIATED BY TUMOR NECROSIS FACTOR-ALPHA (TNF-?). JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2002;4(1):33-37. Available from: https://sid.ir/paper/58796/en

IEEE: Copy

H.A. SHIRZADEH, “NATURAL CYTOTOXICITY (NC) OF IL-3 INDUCED MAST CELL LINE IS NOT MEDIATED BY TUMOR NECROSIS FACTOR-ALPHA (TNF-?),” JOURNAL OF SHAHREKORD UNIVERSITY OF MEDICAL SCIENCES, vol. 4, no. 1, pp. 33–37, 2002, [Online]. Available: https://sid.ir/paper/58796/en

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