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Information Journal Paper

Title

PURIFIED PROTEIN FRACTION OF GARLIC EXTRACT MODULATES CELLULAR IMMUNE RESPONSE AGAINST BREAST TRANSPLANTED TUMORS IN BALB/C MICE MODEL

Pages

  65-74

Keywords

GARLIC (ALLIUM SATIVUM) 
TUMOR INFILTRATING LYMPHOCYTES (TIL) 

Abstract

 Objective: Garlic (Allium sativum) has anti-inflammatory, anti-mutagenesis, and immunomodulatory properties that modulate anti-tumor immunity and inhibit tumor growth. In this study we have examined the effect of a PROTEIN fraction isolated from fresh garlic on anti-tumor response and intra-tumor lymphocyte infiltration.Materials and Methods: In this experimental study a PROTEIN fraction was purified from fresh garlic bulbs using ultra-filtration, followed by chromatofocusing, and SDS-PAGE analysis. Anti-tumor activity was assessed by intra-tumor injection of the PROTEIN fraction and garlic extract, itself, into groups of 5 mice each. The percentage of peripheral blood and intra-tumor CD4+ and CD8+ cells were assessed by flow cytometry. Unpaired student’s t test using the SPSS program was applied for all statistical analyses.Results: Garlic extract included different type of PROTEINs with different molecular weight. One of PROTEIN’s fraction was immunomodeulator and was composed of three single polypeptides, with molecular masses of ~10-13 kDa and different isoelectric points (pI). These molecules augmented the delayed type hypersensitivity (DTH) response compared to the control group. Intra-tumor injection of the fraction provoked a significant increase in the CD8+ subpopulation of T-lymphocytes, as well as a decrease in tumor size. The fraction increased peripheral blood CD8+ T-lymphocytes in treated animals.Conclusion: The data confirms that PROTEIN fractions purified from fresh garlic bulbs augment CD8+ T-cell infiltration into the tumor site, inhibiting tumor growth more efficiently than garlic extract. These fi ndings provide a basis for further investigations on the purified polypeptide as a useful candidate for IMMUNOMODULATION and tumor treatment.

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    Cite

    APA: Copy

    EBRAHIMI, MARZIEH, MOHAMMAD HASAN, ZOHEYR, MOSTAFAEI, ALI, ZARE MEHRJARDI, NARGES, & GHAZANFARI, TOOBA. (2013). PURIFIED PROTEIN FRACTION OF GARLIC EXTRACT MODULATES CELLULAR IMMUNE RESPONSE AGAINST BREAST TRANSPLANTED TUMORS IN BALB/C MICE MODEL. CELL JOURNAL (YAKHTEH), 15(1 (57)), 65-74. SID. https://sid.ir/paper/593505/en

    Vancouver: Copy

    EBRAHIMI MARZIEH, MOHAMMAD HASAN ZOHEYR, MOSTAFAEI ALI, ZARE MEHRJARDI NARGES, GHAZANFARI TOOBA. PURIFIED PROTEIN FRACTION OF GARLIC EXTRACT MODULATES CELLULAR IMMUNE RESPONSE AGAINST BREAST TRANSPLANTED TUMORS IN BALB/C MICE MODEL. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(1 (57)):65-74. Available from: https://sid.ir/paper/593505/en

    IEEE: Copy

    MARZIEH EBRAHIMI, ZOHEYR MOHAMMAD HASAN, ALI MOSTAFAEI, NARGES ZARE MEHRJARDI, and TOOBA GHAZANFARI, “PURIFIED PROTEIN FRACTION OF GARLIC EXTRACT MODULATES CELLULAR IMMUNE RESPONSE AGAINST BREAST TRANSPLANTED TUMORS IN BALB/C MICE MODEL,” CELL JOURNAL (YAKHTEH), vol. 15, no. 1 (57), pp. 65–74, 2013, [Online]. Available: https://sid.ir/paper/593505/en

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