The Effect of Nano-Pyrazole Derivative of Thiosemicarbazone Magnetite on BRCA1, p53 and Bcl-2 Gene Expression in MCF-7 Breast Cancer Cell Line

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

IZADPANAH M.R.; SALEHZADEH A.; ZAEFIZADEH M.; Nikpasand m.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2019 | Volume:19 | Issue:2 Page(s): 227-238

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

489

Download:

Cites:

Information Journal Paper

Title

The Effect of Nano-Pyrazole Derivative of Thiosemicarbazone Magnetite on BRCA1, p53 and Bcl-2 Gene Expression in MCF-7 Breast Cancer Cell Line

Author(s)

IZADPANAH M.R. | SALEHZADEH A. | ZAEFIZADEH M. | Nikpasand m. | Issue Writer Certificate

Keywords

MCF-7 CellsQ1

ThiosemicarbazoneQ1

Fe3o4 NanoparticlesQ1

Breast CancerQ2

Abstract

Background & objectives: Breast Cancer is one of the most important causes of cancer deaths especially in developing countries. However, some synthetic compounds such as Thiosemicarbazone and its metal derivatives can play a very significant role in reducing the death rate from cancer. The aim of this study was to investigate alterations in the expression of BRCA1, p53 and Bcl-2 genes in Breast Cancer cell line. Methods: In this study, the effect of pyrazole derivative of Thiosemicarbazone (T ) as well as T-bound iron oxide magnetite nanoparticles (T/ Fe3o4 np) were investigated on expression changes of Bcl-2, BRCA1 and p53 in MCF-7 Cells. For this purpose, the toxicity of the compounds was first measured using the MTT reaction for 24 hr. Then, the mRNA expression levels were evaluated using real-time PCR reaction. Results: The results showed a high toxicity for the nano-pyrazole derivative of Thiosemicarbazone magnetite so that; the fatality rate for treated Thiosemicarbazone magnetite derivative cells was much higher than the compound without any nanoparticle. Also, real-time polymerase chain reaction analysis of gene expression indicated a high effect of the derivative on increasing the expression levels of BRCA1 and p53 genes and also decreasing the Bcl-2 gene expression of Bcl-2. Conclusion: The use of anticancer compounds like Thiosemicarbazone which bound to iron oxide nanoparticles would increase the toxicity of the anticancer compounds along with enhancment of delivery of them with high solubility to target tissue.

Cites

No record.

References

Cite

APA: Copy

IZADPANAH, M.R., SALEHZADEH, A., ZAEFIZADEH, M., & Nikpasand, m.. (2019). The Effect of Nano-Pyrazole Derivative of Thiosemicarbazone Magnetite on BRCA1, p53 and Bcl-2 Gene Expression in MCF-7 Breast Cancer Cell Line. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 19(2 ), 227-238. SID. https://sid.ir/paper/59562/en

Vancouver: Copy

IZADPANAH M.R., SALEHZADEH A., ZAEFIZADEH M., Nikpasand m.. The Effect of Nano-Pyrazole Derivative of Thiosemicarbazone Magnetite on BRCA1, p53 and Bcl-2 Gene Expression in MCF-7 Breast Cancer Cell Line. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2019;19(2 ):227-238. Available from: https://sid.ir/paper/59562/en

IEEE: Copy

M.R. IZADPANAH, A. SALEHZADEH, M. ZAEFIZADEH, and m. Nikpasand, “The Effect of Nano-Pyrazole Derivative of Thiosemicarbazone Magnetite on BRCA1, p53 and Bcl-2 Gene Expression in MCF-7 Breast Cancer Cell Line,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 19, no. 2 , pp. 227–238, 2019, [Online]. Available: https://sid.ir/paper/59562/en

Related Journal Papers