IN VITRO STUDY ON EFFECTS OF AMIODARONE AND KETOCONAZOLE ON LEISHMANIA INFANTUM

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RAZI JALALI M.H.; BAHRAMI S.; NAJAFZADEH H.; ASADI Z.

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Journal: JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS) Year:2014 | Volume:14 | Issue:3 Page(s): 257-265

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Title

IN VITRO STUDY ON EFFECTS OF AMIODARONE AND KETOCONAZOLE ON LEISHMANIA INFANTUM

Author(s)

RAZI JALALI M.H. | BAHRAMI S. | NAJAFZADEH H. | ASADI Z. | Issue Writer Certificate

Keywords

AMIODARONEQ2

KETOCONAZOLEQ3

LEISHMANIA INFANTUMQ2

IN VITROQ1

Abstract

Background and objectives: The leishmaniases are considered among the major infectious diseases affecting public health in several regions. There are many chemical agents which are effective in treatment of visceral leishmaniasis. But, overall treatment of visceral leishmaniasis is often difficult. Thus, identification of new chemotherapeutic agents is important for treatment of disease. Since targeting of the ergosterol synthesis pathway of Leishmania may be useful therapeutically, the aim of this study was to investigate the effect of alone or in combination of AMIODARONE and KETOCONAZOLE on LEISHMANIA INFANTUM.Methods: To obtain logarithmic promastigotes of L. infantum, the parasites were cultured in BHI medium with FCS 10% together with antibiotics of penicillin and streptomycin and incubated at 24oC. Amastigote forms were obtained in BHI medium supplemented with 20% FCS at pH of 5.5 which incubated in 37oC. L.infantum susceptibility to AMIODARONE and KETOCONAZOLE was evaluated by proliferation of parasites in the absence or presence of these drugs with MTT assay. For evaluation of antiproliferative synergism against promastigotes and axenic amastigotes, fractional inhibitory concentrations (FIC) were calculated. An isobologram curve was constructed too.Results: AMIODARONE produced a marked reduction in the viability of L.infantum promastigotes and axenic amastigotes. On the other hand KETOCONAZOLE induced a dose dependent effect on the parasites proliferation for promastigotes and axenic amastigotes. When the drugs were used in combination, the results indicated clear synergistic as shown by a concave isobologram and FIC value.Conclusion: The present study represents the evidence that the combination of AMIODARONE plus KETOCONAZOLE acts synergistically in controlling L. infantume IN VITRO. It is possible that AMIODARONE could be used in combination with KETOCONAZOLE to combat infection at low doses, thus reducing its side effects such as cardiotoxicity, thyroid dysfunction and pulmonary fibrosis.

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APA: Copy

RAZI JALALI, M.H., BAHRAMI, S., NAJAFZADEH, H., & ASADI, Z.. (2014). IN VITRO STUDY ON EFFECTS OF AMIODARONE AND KETOCONAZOLE ON LEISHMANIA INFANTUM. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), 14(3), 257-265. SID. https://sid.ir/paper/59571/en

Vancouver: Copy

RAZI JALALI M.H., BAHRAMI S., NAJAFZADEH H., ASADI Z.. IN VITRO STUDY ON EFFECTS OF AMIODARONE AND KETOCONAZOLE ON LEISHMANIA INFANTUM. JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS)[Internet]. 2014;14(3):257-265. Available from: https://sid.ir/paper/59571/en

IEEE: Copy

M.H. RAZI JALALI, S. BAHRAMI, H. NAJAFZADEH, and Z. ASADI, “IN VITRO STUDY ON EFFECTS OF AMIODARONE AND KETOCONAZOLE ON LEISHMANIA INFANTUM,” JOURNAL OF ARDABIL UNIVERSITY OF MEDICAL SCIENCES (JAUMS), vol. 14, no. 3, pp. 257–265, 2014, [Online]. Available: https://sid.ir/paper/59571/en

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