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Information Journal Paper

Title

COMPARATIVE STUDY OF THE EFFECT OF LPS ON THE FUNCTION OF BALB/C AND C57BL/6 PERITONEAL MACROPHAGES

Pages

  45-54

Abstract

 Objective: MACROPHAGEs influence their environment and surrounding immune cells as soon as stimulators affect them. Different sources of MACROPHAGEs induce different reactions in their neighboring immune cells, which result in non-uniform immunologic outcomes. In this experimental research, we compare the behavior of peritoneal MACROPHAGEs to lipopolysaccharide (LPS) stimulation from BALB/Cmice as an indicator of a type 2 immune response and from C57BL/6 mice as an indicator of a type 1 immune response.Materials and Methods: In this experimental study, peritoneal MACROPHAGEs prepared from THIOGLYCOLATE stimulated BALB/C and C57BL/6 mice were treated with 1mg/ml LPS. At different time points after LPS treatment, nitric oxide (NO), interferon gamma (IFN-g), interleukin 4 (IL-4), transforming growth factor b1 (TGF-b1), interleukin 17 (IL-17), and interleukin 10 (IL-10) production were measured in the supernatants of all MACROPHAGE cultures. Indoleamine 2, 3 dioxygenase (IDO) and phagocytic activity were analyzed in the different experimental groups. The supernatant effects of LPS-treated MACROPHAGEs on splenocyte proliferation was assessed by the colorimetric method using a 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reagent.Results: According to cytokine analysis, different mouse strains show different cytokine patterns in response to LPS. C57BL/6 MACROPHAGEs produced more IL-17, IL-10, and IFN-g, while BALB/C MACROPHAGEs produced more TGF- b1 and IL-4. There was no significant difference in IDO activity between strains (p≤0.05). BALB/C mice produced more NO inthe first 24 hours after LPS treatment, but C57BL/6 produced more NO at 72 hours post-LPS treatment. MACROPHAGEs from both strains hada suppressor effect on splenocyte proliferation, but this effect was stronger in BALB/C mice.Conclusion: The results show that MACROPHAGEs from different genetic backgrounds respond differently to the same stimulus in aspects of type, intensity, and time of response. The consideration of these aspects will enable researchers to use correct treatment programs for immune-regulation or immunotherapy.

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    APA: Copy

    SOUDI, SARA, ZAVARAN HOSSEINI, AHMAD, MOHAMMAD HASSAN, ZUHAIR, SOLEIMANI, MASOUD, JAMSHIDI ADEGANI, FATEMEH, & HASHEMI, SEYED MAHMOUD. (2013). COMPARATIVE STUDY OF THE EFFECT OF LPS ON THE FUNCTION OF BALB/C AND C57BL/6 PERITONEAL MACROPHAGES. CELL JOURNAL (YAKHTEH), 15(1 (57)), 45-54. SID. https://sid.ir/paper/595754/en

    Vancouver: Copy

    SOUDI SARA, ZAVARAN HOSSEINI AHMAD, MOHAMMAD HASSAN ZUHAIR, SOLEIMANI MASOUD, JAMSHIDI ADEGANI FATEMEH, HASHEMI SEYED MAHMOUD. COMPARATIVE STUDY OF THE EFFECT OF LPS ON THE FUNCTION OF BALB/C AND C57BL/6 PERITONEAL MACROPHAGES. CELL JOURNAL (YAKHTEH)[Internet]. 2013;15(1 (57)):45-54. Available from: https://sid.ir/paper/595754/en

    IEEE: Copy

    SARA SOUDI, AHMAD ZAVARAN HOSSEINI, ZUHAIR MOHAMMAD HASSAN, MASOUD SOLEIMANI, FATEMEH JAMSHIDI ADEGANI, and SEYED MAHMOUD HASHEMI, “COMPARATIVE STUDY OF THE EFFECT OF LPS ON THE FUNCTION OF BALB/C AND C57BL/6 PERITONEAL MACROPHAGES,” CELL JOURNAL (YAKHTEH), vol. 15, no. 1 (57), pp. 45–54, 2013, [Online]. Available: https://sid.ir/paper/595754/en

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