EVALUATION OF THE β-LACTAMASE DISK TEST METHOD IN THE DETECTION OF EXTENDED-SPECTRUM-β-LACTAMASES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA

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KALANTAR NEYESTANAKI DAVOOD; JABAL AMELI FERESHTEH; MIRSALEHIAN AKBAR; EMANEINI MOHAMMAD

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Journal: Journal of Kerman University of Medical Sciences Year:2016 | Volume:23 | Issue:1 Page(s): 1-11

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Title

EVALUATION OF THE β-LACTAMASE DISK TEST METHOD IN THE DETECTION OF EXTENDED-SPECTRUM-β-LACTAMASES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA

Author(s)

KALANTAR NEYESTANAKI DAVOOD | JABAL AMELI FERESHTEH | MIRSALEHIAN AKBAR | EMANEINI MOHAMMAD | Issue Writer Certificate

Keywords

β-LACTAM RESISTANCEQ2

EXTENDED-SPECTRUM-β-LACTAMASES (ESBLS)Q2

CONFIRMATORY TESTSQ2

PSEUDOMONAS AERUGINOSAQ2

BURNQ2

Abstract

Background & Aims: The production of extended-spectrum-b-lactamases (ESBLs) is the main mechanism of resistance to b-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to b-lactam antibiotics caused a decrease in sensitivity of the CONFIRMATORY TESTS for ESBL. The aim of this study was the evaluation of the b-lactamase disk test method in the detection of ESBLs in clinical isolates of multidrug-resistant PSEUDOMONAS AERUGINOSA.Methods: A total of 100 multidrug-resistant P. aeruginosa isolates were recovered from BURN patients. The sensitivity of the isolates to different antibiotics was determined using the standard disk diffusion method. ESBL-producing isolates were detected through the combination disk test with clavulanic acid, double disk synergy test, and b-lactamase disk test. Carbapenemase-producing isolates were detected using the Modified Hodge Test (MHT). The ESBLs genes (blaTEM, blaOXA, blaPER, blaSHV, blaCTX-M and blaPSE) were determined through polymerase chain reaction (PCR).Results: All isolates were multidrug resistant. Only 3 isolates were detected as ESBL-producing isolates through combination disk test. No ESBL-producing isolates were detected through double disk synergy test. Among the 100 studied isolates, 87% were detected as ESBL-producing isolates and 68% as carbpenemase-producing isolates through b-lactamase disk test. The prevalence of blaTEM, blaOXA, and blaPER among isolates were 97%, 61%, and 13%, respectively. All isolates were negative for blaSHV, blaPSE, and blaCTX-M.Conclusion: According to the results obtained in this study, the b-lactamase disk test is suitable for the detection of ESBLs in multidrug resistant isolates. However, further investigation is required.

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APA: Copy

KALANTAR NEYESTANAKI, DAVOOD, JABAL AMELI, FERESHTEH, MIRSALEHIAN, AKBAR, & EMANEINI, MOHAMMAD. (2016). EVALUATION OF THE β-LACTAMASE DISK TEST METHOD IN THE DETECTION OF EXTENDED-SPECTRUM-β-LACTAMASES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, 23(1), 1-11. SID. https://sid.ir/paper/597/en

Vancouver: Copy

KALANTAR NEYESTANAKI DAVOOD, JABAL AMELI FERESHTEH, MIRSALEHIAN AKBAR, EMANEINI MOHAMMAD. EVALUATION OF THE β-LACTAMASE DISK TEST METHOD IN THE DETECTION OF EXTENDED-SPECTRUM-β-LACTAMASES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;23(1):1-11. Available from: https://sid.ir/paper/597/en

IEEE: Copy

DAVOOD KALANTAR NEYESTANAKI, FERESHTEH JABAL AMELI, AKBAR MIRSALEHIAN, and MOHAMMAD EMANEINI, “EVALUATION OF THE β-LACTAMASE DISK TEST METHOD IN THE DETECTION OF EXTENDED-SPECTRUM-β-LACTAMASES IN CLINICAL ISOLATES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA,” JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, vol. 23, no. 1, pp. 1–11, 2016, [Online]. Available: https://sid.ir/paper/597/en

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